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Field
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candidates with experience in one or more of the following areas, cell line editing (CRISPR-KO and knock-in base editing), NK and cytotoxic T cell co-culture assays, single-cell transcriptomics, HLA
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approaches such as non-coding CRISPR screens, the Massively Parallel Reporter Assay (MPRA), saturation mutagenesis, and synthetic sequence design, alongside machine-learning models of regulatory grammar
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. The successful candidate will help prioritize, validate, and develop therapeutic hypotheses generated from large cancer datasets, with opportunities to lead projects spanning CRISPR screening, drug-response
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/ lentiviral transduction, transposition, CRISPR-Cas9). Viral particle assembly. Excellent aseptic cell culture technique. Enthusiasm and a willingness to learn in a fast-pace, collaborative environment. Strong
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techniques: identifying the factors that establish early RT at target genes, determining how they promote translocation biogenesis, and testing them in preclinical models. The work combines CRISPR engineering
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hepatocytes, CRISPR/Cas9 gene editing, and genetically distinct HBV clones to dissect the host and viral factors that determine infection and persistence. Recent work from the laboratory, published in Cell
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The University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 2 months ago
measurements of mucus transport, which may include particle transport assays and optical coherence tomography. Related work may include single-cell RNA/ATAC sequencing, RNA in situ hybridization, and CRISPR/Cas9
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) Familiarity with molecular cloning, viral vectors, or CRISPR-based gene editing Prior research in cerebrovascular or neurological diseases Appointment and Location: The position is based at the Anlyan Center in
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-on experience with CRISPR-Cas genome editing, including generation and validation of knockout (KO) lines. Expertise in base- and prime editing, endogenous gene tagging and targeted knock-in of large DNA cargos
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malignancy. The project integrates mouse and iPSC models, CRISPR genome editing, molecular biology, flow cytometry, and single-cell multi-omics to dissect disease mechanisms. We are committed to mentoring