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Field
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of DNAAF9 (axonemal dynein assembly factor 9) in ciliary biology. This will involve CRISPR genetic engineering using relevant ciliated cell lines to knockout DNAAF9 followed by cytological, proteomic and
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methodologies: flow cytometry, genome engineering (CRISPR, CRISPR-interference), high-throughput screening in cells, mammalian cell culture, Independence and the ability to work in a team, Motivation and
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animal models for hematopoiesis Experience in high-throughput data analysis, such as single cell RNA sequencing Knowledge in R and foundations of statistics Experience with CRISPR/Cas9 (or similar) genome
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laboratory at the Waksman Institute of Microbiology has an open Postdoctoral Associate position to investigate CRISPR-Cas13-mediated immunity. Under the supervision of Dr. Semenova, the postdoctoral fellow
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-learning approaches. Projects may also include detailed molecular and functional characterization of particularly exciting candidate evolutionary changes using organoid and mouse models, CRISPR-based
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything
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to genome-scale analysis. The following qualifications are strongly preferred: Experience culturing primary human endothelial cells. Experience with CRISPR-based functional assays or other molecular
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will combine CRISPR/Cas9-mediated gene editing, perturbation screening, high-end flow cytometry, functional immune assays and single-cell multiomics to identify cellular and molecular determinants
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differentiation (ideally mouse ESCs), single cell sequencing, method development, flow cytometry/FACS, and/or CRISPR-based perturbations, data analysis. Theoretical background in epigenetics and embryonic
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candidates with experience in one or more of the following areas, cell line editing (CRISPR-KO and knock-in base editing), NK and cytotoxic T cell co-culture assays, single-cell transcriptomics, HLA