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Field
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hepatocytes, CRISPR/Cas9 gene editing, and genetically distinct HBV clones to dissect the host and viral factors that determine infection and persistence. Recent work from the laboratory, published in Cell
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The University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | about 2 months ago
measurements of mucus transport, which may include particle transport assays and optical coherence tomography. Related work may include single-cell RNA/ATAC sequencing, RNA in situ hybridization, and CRISPR/Cas9
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and Pacific Biosciences platforms, single-cell multi-omics, 3D genome architecture studies (e.g., Micro-C and HiC), CUT&RUN and CUT&Tag. Experimental systems will include CRISPR/Cas9-mediated genetic
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-on experience with CRISPR-Cas genome editing, including generation and validation of knockout (KO) lines. Expertise in base- and prime editing, endogenous gene tagging and targeted knock-in of large DNA cargos
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Research Associate. Our lab specializes in precision cardiovascular medicine, leveraging cutting-edge approaches such as human iPSC-derived 2D cardiovascular cells, 3D vascularized cardioids, CRISPR gene
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the other postdoc included in the project. Main tasks of the postdoc Culture M. leidyi and use CRISPR-cas9 to obtain knockout strains Perform common garden experiments testing wildtype vs knockout strains
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. Joost Smolders at MS Center ErasMS in Rotterdam. The project combines functional genetics in primary human B cells and B cell lines (CRISPR-KO and knock-in base editing) with immunological co-culture
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-based methods such as RNA-Seq and ChIP-Seq is expected, Experience in microscopy, scRNA-Seq, FACS, and CRISPR-based cell line engineering is desirable but not required; candidates without prior experience
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plasticity in Leishmania, within the context of a FAPESP Young Investigator Grant. The work will involve the generation and characterisation of mutant lines using CRISPR/Cas9 and DiCre, analysis of cell-cycle
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proliferative). • Investigate how ECM composition and mechanical properties regulate melanoma cell states and behaviour using CRISPR-Cas9-mediated perturbations, advanced imaging approaches, and physiologically