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Field
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. Mutations and deletions of NR2F1 lead to Bosch-Boonstra-Schaaf optic atrophy syndrome (BBSOAS). Using neuronal cells derived from CRISPR/Cas9 engineered human induced pluripotent stem cells (hiPSC) we model
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University of California, Los Angeles | Los Angeles, California | United States | about 2 months ago
, time-management, and collaboration skills are required. Preferred qualifications • Molecular & Synthetic Biology: Extensive experience in molecular cloning, genetic circuit design, CRISPR/Cas systems
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engineering techniques (knockdown; knockout with Crispr/cas9 and/or base editors) , myeloid cell differentiation from inducible pluripotent stem cells and functional assays of immune regulation, inflammasome
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engineering (CRISPR/Cas9, Gal4/UAS), including the generation of genetic tools in non-model Drosophila species; single-cell genomics (scRNA-seq and single-cell ATAC-seq); in vivo functional imaging (two-photon
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 3 months ago
reporter assays (MPRA) and CRISPR-based screens. The individual will use human induced pluripotent stem cells (hiPSCs) and mouse models to generate genomic libraries, perform sequencing-based experiments
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protein biochemistry, molecular biology, proteomics, and cell biology perform mechanistic studies in mammalian cell systems and mouse models design and implement CRISPR/Cas9-based genetic manipulation
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experimental approaches such as non-coding CRISPR screens, the Massively Parallel Reporter Assay (MPRA), saturation mutagenesis, and synthetic sequence design, alongside machine-learning models of regulatory
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laboratory experiments for CRISPR, molecular biology, genetics, and related experiments, • Prepare samples for DNA/RNA extractions, PCRs, qPCRs, library prep for Illumina and long read sequencing, and other
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-derived models. Performs CRISPR/Cas9-mediated gene knockout (KO), siRNA/shRNA-mediated knockdown (KD), and validation of engineered cell lines. Conducts the multi-omics data analysis including RNA-seq
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amplification, RT-PCR amplification, TC culture, BLS2/3 work, CRISPR/Cas9 screening and editing, and analysis of results Data Compilation Recover, compile and verify accuracy of research data, Interpret and