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Field
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. Experience using CRISPR/Cas9 systems to translate structural findings into mammalian cell-based assays, a strong background in E3 ubiquitin ligases, and the use of biophysical techniques to investigate protein
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, qPCR, cloning, CRISPR/Cas9, Hi-C, or related methods). Experience in human genetics, rare disease research, or chromosome biology. Experience with functional validation of candidate genes or variants
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range of genomic and molecular biology approaches, from developing epigenetic insulator systems, epigenome profiling and CRISPR-based epi-engineering, to long-read sequencing, karyotyping, and live-cell
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Research Center for Molecular Medicine (CeMM), ÖAW | Vienna, Virginia | United States | 2 months ago
CRISPR perturbation profiling. Tissue immunology, immunotherapy, cell engineering, and/or synthetic biology (Bock Lab , CeMM/MedUni Vienna). We develop and apply innovative technologies for analyzing
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and investigation into the biology of novel therapeutic targets in pediatric bone sarcomas. Dr. Guenther's research utilizes genome-scale CRISPR screening and other complementary large-scale chemical
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Sciences/Engineering, or a related field. In addition to the required expertise, preferred skills include: mammalian cell culture, molecular cloning, flow cytometry, CRISPR/Cas9 based genetic perturbations
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adult offspring immune organs, followed by sample processing and downstream analyses. Perform mammalian cell culture, transfection, CRISPR-Cas9 gene editing and clone validation, flow cytometry, immune
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-epithelial interactions. The role employs a multi-modal research approach spanning genetically engineered mouse models, histopathology, mouse and human organoids, CRISPR-Cas9 gene editing, single-cell
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) • Differentiation and culture of human pluripotent stem cells toward hematopoietic and endothelial fates, organoid assays and generation of iHSC or other hematopoietic lineages. • Generate and characterize CRISPR
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chromatin accessibility, CRISPR based gene and epigenetic perturbation, and deeply phenotype human cohorts to define causal mechanisms driving post infectious and post tuberculosis lung disease. Postdoctoral