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. The successful candidate should have extensive experience from experimental laboratory work in molecular cell biology using techniques such as cell culture, CRISPR/Cas9 based genome editing, protein expression and
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infection and inflammation. The successful candidate will lead and contribute to research projects using molecular and cell biology, biochemistry, cell culture, viral infection models, and mouse models
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, fluorescence microscopy and/or bioluminescence experiments applied to cell biology Basic cell-culture skills; experience with cellular differentiation models is an asset Strong project organisation
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disorders and aggressive cancers. Experimental approaches to be applied will include culture and transfection/infection of mammalian cell lines including CRISPR gene editing, protein structure function
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/science.aea5113) Stockis J et al. Science Immunology 2024 (DOI: 10.1126/sciimmunol.adl1903) Schuijs MJ et al. Nature Immunology 2020 (https://doi.org/10.1038/s41590-020-0745-y ) Preferred skills/knowledge Cell
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of the “Cell Signaling and Pathogenesis” team: https://cimiparis.fr/equipes/robert-weil/ Where to apply E-mail [email protected] Requirements Research FieldBiological sciences » BiologyEducation
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-relevant models that link molecular and cellular findings to disease mechanisms and clinical relevance. The work includes advanced cell culture of various cardiovascular cell types, 3D cell models, spheroids
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contribute to a project involving the development of therapeutic molecules. Description of the activities Ensure the culture of adherent cell lines (BS2) Perform cytotoxicity tests Perform IP1 (inositol
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human endothelial cell culture and perturbation systems with genomic discovery tools to dissect how genetic variation at CAD-associated loci operates through the vessel wall. Active research areas include
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techniques in cell culture (routine cell maintenance, transient transfections, preparation of nuclear extracts, utilization of CRISPR/Cas9) Assist with fluorescent imaging techniques, both biochemical and in