Research engineer to MTC

The Department of Microbiology, Tumor and Cell Biology (MTC) at Karolinska Institutet conducts research and teaching within immunology, infection biology, cell biology and cancer. MTC has about 40 research groups and our key words are multidisciplinary, bridging, national and international collaborations. We are looking for research engineer within Single Cell Cancer Genomics to Bienko-Crosetto Lab.

You will be part of a collaborative project aimed at characterizing the genetic heterogeneity of Luminal B breast cancers, by applying a single-cell DNA sequencing technique already established in our lab to breast cancer biopsies available from our clinical collaborator at Karolinska University Hospital.

You will be embedded in a dynamic research environment focused on basic genome biology, molecular methods development and oncogenomics. You will be working alongside senior scientists, postdocs and PhD students from different fields and diverse cultural backgrounds.

Your main tasks will be:

• Preparing single-nuclei suspensions from frozen biopsies.
• Performing sorting of the nuclei into 384-well plates at the Sorting Facility at Biomedicum.
• Performing ACT (Minussi et al, Nature 2021) on the sorted nuclei by leveraging a liquid nanodispensing device (I.DOT) available in our lab.
• Pre-processing the raw sequencing data and performing DNA copy number calling using well-established computational pipelines available in our lab.

You will work alongside a clinical postdoc and a PhD student expert in bioinformatics who will be responsible for your daily supervision.

We are looking for you who are passionate about single-cell genomic technologies and want to contribute to research on a tumor type that still has a major socio-economic burden. We are searching for you who are meticulous and attentive to details.

• You must hold a master’s degree in biotechnology/biomedicine/bioengineering or equivalent, obtained no earlier than 2021.
• Have a documented experience in working in the lab on one or more genomic techniques.
• Prior hands-on work with single-cell DNA sequencing methods is a major advantage.

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Solna

More information about the Magda Bienko Group

More information about the Bienko-Crosetto lab

Welcome to apply at the latest2023-02-03

The application is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form.