10 Apr 2024
Job Information
- Organisation/Company
Institute of Fundamental Technological Research Polish Academy of Sciences- Department
Department of Biosystems and Soft Matter- Research Field
Biological sciences- Researcher Profile
Recognised Researcher (R2)- Country
Poland- Application Deadline
8 May 2024 - 23:59 (Europe/Warsaw)- Type of Contract
Temporary- Job Status
Full-time- Hours Per Week
40- Offer Starting Date
1 Jun 2024- Is the job funded through the EU Research Framework Programme?
Not funded by an EU programme- Is the Job related to staff position within a Research Infrastructure?
No
Offer Description
Project title: Understanding multi-scale innate immune defences against bacterial pathogens at the single-cell level
Founded by the National Science Centre
We are seeking enthusiastic and highly motivated scientists to join a newly established laboratory of Prof. Pawel Paszek (founded via a PLN 2m OPUS-23 grant from NCN and PLN 1.6m Polish Returns grant from NAWA https://nawa.gov.pl/nawa/aktualnosci/nawa-polskie-powroty-nabor-rozstrzygniety ). Successful candidate will work in a team of 5 interdisciplinary scientists using interdisciplinary approaches to study single cell immune responses to bacterial infections.
The main aim of the project is to quantitatively understand mechanisms involved in the muti-scale coordination of innate immune responses against bacterial pathogens at the single cell level. We will study direct interactions between innate immune macrophages and the important food-borne bacteria Listeria monocytogenes that causes significant levels of human morbidity and mortality. Our data demonstrate that interactions between L. monocytogenes and macrophages, a key event controlling the overall infection critically rely on a small fraction of ‘successful’ pathogens, which can effectively replicate and spread within the infected host cell population.
Our main objectives are:
- Elucidate via time-lapse live-cell imaging how pathogen threats are encoded in dynamical responses of NF-kB/STAT/IRF systems upon L. monocytogenes infection.
- Understand the population-level host strategies that underpin successful immune response to infection. We will investigate how paracrine signalling in the infected and bystander population and temperature adaptation coordinate the overall population level inflammatory responses.
- Determine whether the host and pathogen variability are co-regulated as a fundamental immune mechanism. We will use image genomics (smFISH) and immunostaining to measure single-cell effector responses in host macrophages as well as monitor activation of the major PrfA regulon in L. monocytogenes at the single bacterium resolution.
- Develop predictive mathematical models of innate immune response to L. monocytogenes using genetic and chemical perturbations to validate modelling and manipulate infection outcomes.
Requirements
- Research Field
- Biological sciences
- Education Level
- PhD or equivalent
Skills/Qualifications
We seek excellent candidates having experience and publication record in one of the following areas:
Background in both areas will be considered beneficial.
Specific Requirements
Candidates will be expected to use following approaches in project (training will be provided):
- Live-cell confocal imaging using L. monocytogenes encoding fluorescent proteins and reporters of Prfa virulence, human and mouse cells (primary as well as immortalized macrophage lines) expressing fluorescently tagged transcription factors and cytokines.
- Immunostaining. Simultaneous measurement of regulatory proteins involved in regulation of immune responses in single cells. We will also use smFISH and the emerging techniques of sequential immunostaining.
- Gene expression, protein analysis and cytokine production in infected cell populations through the use of RT-PCR, digital PCR, Western Blotting, ELISA, generation of reporter lines using molecular cloning and CRISPR, and other relevant techniques.
- Microfluidic systems to culture adherent and suspended cells allowing for their confocal imaging and immunostaining.
- Mathematical modeling employing Markov processes to describe activation of the immune response in heterogeneous cell populations, cellular communication, density and temperature effect, different outcomes of infection (eradication, persistence, replication), regulation of bacterial virulence. We will account for spatial aspects of the cytokine diffusion and bacterial spread.
- Languages
- ENGLISH
- Level
- Excellent
- Research Field
- Biological sciences
- Years of Research Experience
- 4 - 10
Additional Information
Benefits
Eligibility criteria
Candidates must hold a PhD degree in a relevant field and have excellent track record for their career stage.
Selection process
Please provide the following documentation with your application:
1. Copy of PhD diploma in relevant field. MSc and Bachelor diplomas may be also included for evaluation.
2. CV containing information about:
- Publications,
- Conference presentations,
- Prizes and stipends,
- Other information that can help in evaluation, like experience, achievements, and scientific standing of the candidate.
- Contact details for two references,
- Please include in your CV the following clause: "I agree to the processing of personal data contained in my job offer for the needs necessary to carry out the recruitment process conducted by IPPT PAN with headquarters in Warsaw, ul. A. Pawińskiego 5B, according to art. 13 para. 1 and 2 of Regulation (EU) 2016/679 of the Parliament and of the Council of 27 April 2016 on the protection of individuals with regard to the processing of personal data and the free movement of such data and the repeal of Directive 95/46 / EC (RODO).
3. Motivation letter (1 page).
In your application, please indicate the competition number BSP/DSP/110-51/2023
The documents should be sent till to [email protected] , with a copy to Project PI, Pawel Paszek ([email protected]) and Department Secretary Ms. Agnieszka Ponarska ([email protected] ). Suitable candidates will be contacted and invited for an interview.
Work Location(s)
- Number of offers available
- 1
- Company/Institute
- Institute of Fundamental Technological Research Polish Academy of Sciences
- Country
- Poland
- State/Province
- Mazovia
- City
- Warsaw
- Postal Code
- 02-106
- Street
- A.Pawińskiego 5B
Where to apply
[email protected]
Contact
- State/Province
Mazovia- City
Warsaw- Website
http://www.ippt.pan.pl- Street
A.Pawińskiego 5B- Postal Code
02-106
[email protected]
STATUS: EXPIRED
Similar Positions
-
Fachbereich Biologie, Chemie, Pharmazie Institut Für Chemie Und Biochemie Research Assistant (Praedoc) (M/F/D) With 70%Part Time Job Limited For Up To 4 Years Salary Grade (Entgeltgruppe) 13 Tv L Fu Reference Code: Freund Palmitoylation Trr186 Bewerbungsende: 08.07.2024, Free University of Berlin, Germany, about 13 hours ago
A PhD student position is available in the Freund group at FU Berlin (https://www.bcp.fu-berlin.de/en/chemie/biochemie/research-groups/freund-group/index.html ) for 4 years in the project “The rol...
-
Scientist (M/F/D) “Regulatory Affairs Cell Line Banking And Customer Support”, Leibniz, Germany, about 23 hours ago
Scientist (m/f/d) “Regulatory Affairs Cell Line Banking and Customer Support” at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany, in th...
-
Scientific Research Technician Cancer Stem Cells (Veterinary Faculty), AcademicTransfer, Netherlands, about 7 hours ago
We are looking for a scientific research technician who will be providing technical support to our research team. You will be working closely with 2 PhD candidates and with Master's students. You ...
-
Bioinformaticians Biodiversity Cell Atlas Database, Centre for Genomic Regulation, Spain, about 14 hours ago
The Institute The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. T...
-
Qc Associate Ii Gmp Manufacturing, City of Hope, United States, 1 day ago
Join the forefront of groundbreaking research at the Beckman Research Institute of City of Hope , where we're changing lives and making a real difference in the fight against cancer, diabetes, and...
-
Research Associate Ii T Cell Therapeutics Research Laboratory, City of Hope, United States, about 24 hours ago
Thank you for your interest. Please note, the purpose of this posting is to recruit for on-going and future positions. Join the forefront of groundbreaking research at the Beckman Research Instit...