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Field
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autoimmune disease characterized by the loss of immune tolerance toward pancreatic beta cells. This project focuses on the mechanisms underlying tolerogenic responses in T1D by reconstructing in vitro 3D islet
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tissue electrical polarisation influence cell–matrix interactions during lung fibrosis progression. The postholder will develop bioelectronic in vitro platforms to precisely control matrix deformation and
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include expressing recombinant proteins in insect, mammalian, and E. coli cells; purifying proteins using FPLC systems; performing in vitro RNA transcription and labeling; carrying out in vitro assays
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automated liquid-handling workflows on an Opentrons Flex platform (Python API). Establish and run spatial proteomics on in vitro co-cultures, engineered 3D tumor models and tissue sections. Implement clonal
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in vitro using patient source prostate cancer biopsies, cell culture experiments including cytotoxicity assays, uptake studies and molecular biology techniques. • In vivo testing using animal models
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Institut de Recerca Biomèdica de Lleida, Fundació Dr. Pifarré (IRBLleida) | Spain | about 2 months ago
well as the performance of behavioral tests to evaluate motor activity. o The design and execution of both in vivo and in vitro experiments. o The application and development of cell and molecular biology techniques. Where
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cells perform over time in controlled in vitro settings, biomaterial-supported culture environments, and prototype device-relevant configurations. The position requires a technically mature researcher who
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individuals report directly to Dr. Lijuan Yuan. Duties will involve performing flow cytometry to study T cell responses induced by rotavirus and norovirus vaccines in gnotobiotic pigs and other in vitro and in
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production in bioreactors; - The fellow will also carry out physical and biochemical characterization of the recombinant OMVs and evaluate their immunogenic potential in in vitro cellular assays. Mandatory
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at the interface of dentistry and medical microbiology. The goal is to establish and expand an oral biofilm engineering platform, focusing on innovative in vitro and ex vivo models (e.g. dynamic