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Field
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(BSCB) assembloids, and organ-on-a-chip platforms. Execute molecular protocols including qPCR, DNA/RNA extractions, molecular cloning, CRISPR/Cas9 manipulations, Western blotting, tissue sectioning
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will be the functional characterisation of non-coding regulatory elements in bone and cartilage cells. The successful candidate will use CRISPR-Cas9 genome editing in human cell models, including
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alterations impact human lymphocyte development. By combining CRISPR-based genome engineering, induced pluripotent stem cells (iPSCs), and advanced thymus and bone marrow organoid systems, we study both
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. The successful candidate should have extensive experience from experimental laboratory work in molecular cell biology using techniques such as cell culture, CRISPR/Cas9 based genome editing, protein expression and
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– signaling – proteasome – ubiquitination – multiple myeloma – mantle cell lymphoma – cancer – immunology – inflammation – infection – streptococcus – CRISPR/Cas9 – proteomics – targeted therapy Website
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involves the use of diabetic mouse models, isolated primary islets, and stem cell-derived islets, employing techniques such as fluorescence microscopy, proteomics, RNAseq, CRISPR-Cas9, and genetically
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therapeutic editing strategies. Research Focus Areas Neurodevelopmental disorders and the genetic and epigenetic mechanisms underlying altered neural development. Genome and epigenome editing (e.g., CRISPR
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cytometry and cell sorting is also required together with experience of advanced genome editing including cloning of CRISPR gRNAs for custom screens, base editing (including alternative specificity Cas
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. Generate genetically modified cell lines using CRISPR/Cas9 genome editing. Produce lentiviral vectors and perform lentiviral transductions. Design and construct plasmids using standard molecular cloning
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: General reproductive biology experience CRISPR/Cas9 experience Electroporation experience Reproductive immunology background, particularly ovarian or uterine tissue Primary cell culture experience Histology