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USDA-ARS Molecular Biology Postdoctoral Fellowship in the Natural Products Utilization Research Unit
sequence databases, analysis of gene function via CRISPR/Cas-9-mediated genome editing and RNAi, heterologous protein expression, real-time qRT-PCR, and plant transformation using species such as Arabidopsis
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: Experience with lactic acid bacteria or or food-grade yeast species is preferred. Experience with recombinant DNA technologies (i.e. cloning, CRISPR), ethanol fermentation and distillation, or an understanding
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mechanisms in leukemia and other hematologic malignancies. Perform molecular and cellular assays, which may include cell culture, flow cytometry, lentiviral transduction, CRISPR-based perturbation, qRT-PCR
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limited to the following: Design and execute advanced wet laboratory experiments, including human primary epithelial cell culture, cell differentiation, virus handling (BSL2), CRISPR knockout assays
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one or more of: Directed differentiation of pluripotent stem cells Direct cell reprogramming, including derivation of iPSCs Genome editing (e.g. CRISPR, recombinases, targeted integration systems) Gene
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Signal transduction · Experience with standard biochemical, molecular and cellular biology techniques, including gene expression analysis, cloning, CRISPR-based approaches, protein biochemistry
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University of North Carolina at Charlotte | Charlotte, North Carolina | United States | about 15 hours ago
in real time. Our multidisciplinary approach integrates high-resolution imaging with cutting-edge genomics (CUT&RUN, Micro-C, Hi-C), proteomics, and CRISPR/Cas9 engineering to address the most pressing
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, spatial transcriptomics, CRISPR technologies) paired with in vivo animal model systems and human patient-derived biospecimens (organoids and T cells). The candidate should have a strong background in
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fundamental molecular biology techniques (such as gel electrophoresis, Western blotting, qPCR, molecular cloning, CRISPR editing, plasmid construction, virus packing, iPSC culture, RNA-sequencing, multi-omics
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culture and functional assays Molecular biology techniques CRISPR or genetic perturbation approaches Genomics, transcriptomics, or single-cell technologies Pharmacology and drug response studies