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. An additional focus of the group is the study of chromatin organization and 3D genome folding in somatic, stem, and cancer cells, using an integrated and innovative methodology that combines state-of-the-art
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experience with advanced human cell culture, including 3D models and organ-on-chip systems. Hands-on experience with pluripotent stem cell culture. Proficiency in standard molecular and cell biology techniques
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research laboratory setting with state-of-the-art facilities to study the cellular and molecular heterogeneity of esophageal mucosa. The laboratory technician will perform single cell RNA-sequencing, 3D
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Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S) | Portugal | about 2 months ago
cell-matrix communication in human-relevant experimental settings. To achieve this, we will develop bioengineered platforms that combine cell-laden bioinstructive hydrogels, advanced 3D culture systems
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imaging in 2D or 3D cell culture models; electrophysiology and 3D neuronal culture. Experience in data analysis and/or statistical methods is helpful. Equipment Utilized Biosafety cabinets, liquid nitrogen
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composed of a highly interdisciplinary and international team of motivated researchers. To expand on our ability to engineer viscoelastic biomaterials and deploy them as tailorable 3D cell culture platforms
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/ . The successful candidate will receive hands-on training in isolation and culture of different primary human cells from patient lung tissues, and in differentiation of these cells into 2D and 3D tissue-like
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and Pacific Biosciences platforms, single-cell multi-omics, 3D genome architecture studies (e.g., Micro-C and HiC), CUT&RUN and CUT&Tag. Experimental systems will include CRISPR/Cas9-mediated genetic
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processing, cell and bacterial culture, and imaging workflows underpinning studies of disease progression and treatment response. This work sits within a wider programme applying tissue-engineered, in vivo
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, microscopy, and molecular analysis. Some experiments may require careful timing, serial sample collection, or occasional scheduling flexibility. 40% — Human cell culture, co-culture systems, and 3D skin model