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Field
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Gram negative bacteria such as E. coli use two main mechanisms to survive in the presence of a range of toxic molecules such as antibiotics, biocides, and solvents. First, their envelope prevents
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investigate the impact of post-transcriptional modifications of transfer RNAs (tRNAs) on stress tolerance and adaptation in clinical strains of *Escherichia coli*. The project will combine experimental
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In the research group of Prof. Dr. Lydia Herzel (RNA Dynamics) a doctoral position is to be filled to work on transcription dynamics in E. coli and to characterize the contribution of a number of
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*, Shigella*, and pathogenic E. coli. The group is interested in defining the mechanisms of pathogenesis and the mechanisms of plasmid biology with the long-term aim of applying these findings
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protein design (Chemical Science 13, 11330-11340, (2022); Nature Chemical Biology 20, 991-999 (2024)). Designs will be produced by recombinant methods in E. coli, and characterised using a variety of
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experience with mammalian cell culture, protein expression (E. coli, yeast, and mammalian cells) and purification, and/or Drosophila genetics, including dissection, immunostaining, and confocal microscopy
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(Chemical Science 13, 11330-11340, (2022); Nature Chemical Biology 20, 991-999 (2024)). Designs will be produced by recombinant methods in E. coli, and characterised using a variety of techniques with which
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are needed to prevent infections with bacterial enteropathogens and AMR. Our previous research showed that there are differences in the E coli colonization of infants’ microbiome between LMIC vs. high-income
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Scientist will participate in studies evaluating engineered E. coli Nissle strains designed to restore microbiome-derived metabolites, including GABA and butyrate, and determine their effects on
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person to join our team. Core topics to work on: (1) In vitro assays to determine inhibitor efficacy against redox membrane proteins (2) Cysteine enrichment of DsbA substrates in E. coli and Mycobacteria