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person to join our team. Core topics to work on: (1) In vitro assays to determine inhibitor efficacy against redox membrane proteins (2) Cysteine enrichment of DsbA substrates in E. coli and Mycobacteria
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against E. coli, in order to guide vaccine development. We seek a motivated clinical research fellow to play a pivotal role undertaking an observational clinical cohort study at the Oxford University
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Responsibilities: Design and engineer novel recombinant proteins with tailored mechanical or functional properties. Manage high-yield protein expression (E. coli or alternative hosts) and multi-step chromatographic
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Center for Drug Evaluation and Research (CDER) | Silver Spring, Maryland | United States | about 3 hours ago
, HeLa) and prokaryotic (e.g., E. coli) cell extracts to systematically assess their viability and reproducibility for safe and effective biomanufacturing. Research Initiatives: Improve or advance science
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topics to work on: (1) In vitro assays to determine inhibitor efficacy against redox membrane proteins (2) Cysteine enrichment of DsbA substrates in E. coli and Mycobacteria using proteomics. (3) determine
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macrophages, neutrophils and T cells Cytokine biology and immune signaling pathways (e.g. G-protein signaling) Pathogens of interest include E. coli , S. pneumoniae , Legionella spp., and others. Projects will
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recombination and phenotypic screening. Experience in use of molecular biology techniques for the cloning of genes via E. coli the expression and purification of proteins of interest for in vitro enzyme assays
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Expertise: Extensive hands-on experience with method development for protein expression and purification (specifically recombinant protein production in E. coli). Biophysical Techniques: Demonstrated
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hands-on work using E. coli as a model organism. Experience in techniques such as molecular cloning and genetic engineering is expected. Prior experience with directed evolution approaches, including
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to make the synthesis of model organism genomes (i.e. E. coli) more rapid and enable the synthesis of the genomes of non-model bacteria to broaden the scope of genome synthesis. The ability to routinely