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, chemistry, biology, etc.) Knowledge and experience in molecular biology The following skills would be an advantage: establishing bioassays, cloning, protein expression, working with cell cultures, E. coli and
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the evolution, cell biology, and virulence of bacterial pathogens, including Vibrio cholerae , enterohemorrhagic Escherichia coli , Shigella , Salmonella spp ., and Listeria monocytogenes . They take
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proteins in E. coli and insect cells, and, where required, in mammalian cell culture. • Purification of proteins and protein complexes using a range of chromatographic methods (ÄKTA systems
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, protein expression (e.g. E. coli), protein purification and biochemical assays Experience working in a laboratory environment with a strong understanding of biosafety and health & safety practices Excellent
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multiple systems: bacterial (E. coli), mammalian (HEK293, HaCaT, A431), and insect cells (Sf9, baculovirus production). Purify challenging targets using ÄKTA SEC and affinity columns, perform sucrose
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with bacteriophages, E. coli and yeast. We seek a Staff Scientist to conduct experiments on molecular evolution under the supervision and guidance of the Principal Investigator, including assisting in
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works primarily with bacteriophages, E. coli and yeast. We seek a Staff Scientist to conduct experiments on molecular evolution under the supervision and guidance of the Principal Investigator, including
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Proteomics and Cell assays. Candidates with mostly Synthetic/Medicinal Chemistry training are discouraged from applying. Experience in protein purification from E. coli will be considered favorably, but not
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. Experience working beyond standard bacterial model systems (e.g. E. coli) is highly desirable. Prior experience in environmental microbiology is not required. Our lab specializes in high-throughput
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to identify novel OM influx and efflux processes in gut Bacteroides via a multi-disciplinary approach to understand why this genus is so abundant. Compared to Proteobacteria like E. coli in which