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research duties to support the laboratory of Dr. John Rawls (https://sites.duke.edu/rawlslab/ ). Dr. Rawls’ lab uses zebrafish, mice, and bacterial model systems to investigate mechanisms of host-microbiome
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immunoassays: perform flow cytometry (sample preparation, staining, acquisition and analysis) and enzyme-linked immunosorbent assays (ELISA); Traceability and protocols: compile, format and record experimental
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, molecular and cell biology and have experience with basic molecular biology techniques (RNA and DNA analysis, PCR, RT-qPCR, protein analysis, Western immunoblotting, and ELISA), flow cytometry, mass cytometry
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Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences | Poland | about 1 month ago
. Proficiency in flow cytometry techniques, enzyme-linked immunosorbent assays (ELISAs), including multiplex methods, the assessment of cytotoxicity using various methods, and fluorescence microscopy
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cells. · Conducting in vitro experiments including flow cytometry, qPCR, ELISA. Preference given to candidates with prior familiarity with these tools. · Performing in vivo experiments in
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, immunohistochemistry, immunofluorescence, ELISA, flow cytometry, Western blot, quantitative PCR, molecular cloning, and miRNA analyses. Also, the Researcher will be responsible for assisting other individuals in the lab
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, and protein chemistry procedures. These routine lab procedures include microscopy, histology, immunohistochemistry, immunofluorescence, ELISA, flow cytometry, Western blot, quantitative PCR, molecular
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colorimetric and fluorescent assays ELISA Flow cytometry Fluorescence and confocal microscopy General knowledge of working with primary and cancer cell lines is also required. The position also includes
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analysis and familiarity with R, python or another programming language Experience in molecular biology techniques including immunohistochemistry, qPCR, cell culture, ELISAs Software experience including
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culture, and iPSCs, molecular cloning, RNA/DNA purification, protein extraction, immunohistochemistry, Western blot analysis, and ELISA is desired. * Ability to operate all standard laboratory and office