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will be focused on the following projects in MCTP: ? The focus of study for this position will be performing cell-free extractions of DNA and RNA fragments from proteins and other cellular
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extraction. Animal tissue dissections, tissue fixation and sectioning, immunohistochemical staining. Single cell suspensions from isolated tissue, flow cytometry, RNA isolation, RT-qPCR, ELISA. Analyzing data
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provides approximately 2.3 million clinic visits and 54,000 surgeries per year. Data for research is extracted from our Electronic health record (EHR) data and numerous other data sources into our Research
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microscopy; and image analysis using software such as ImageJ. Maintaining primary 2D and 3D cell culture, single cell extraction from tissue, RNA/Protein isolation, qRT-PCR, Western blot, flow cytometry, and
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of IC50, perform proliferation assays etc. Functional assays: Perform protein and RNA extraction from cells and tumor tissue, western blot, co-immunoprecipitation, qPCR, ELISA assay, gDNA extraction, T7