PhD student position: deciphering the molecular basis of allostery in CRISPR associated proteins using single-molecule experiments

University of Basel, Switzerland

Updated: about 1 month ago

Your position

This PhD project will grow with you. You will create time-resolved direct observations of single CARF proteins – aka. CRISPR suicide proteins – undergoing allosteric activation, substrate processing, deactivation, etc. How this works, on what timescales, by which conformational rearrangements has not been observed yet in real-time, leaving the underlying molecular processes obscure. In this PhD project, you'll be able to solve (some of) these nanoscale puzzles. Steps involved in the project:

  • Single-molecule fluorescence/FRET/DyeCycling experiments
  • Solid-state nanopore/NEOtrap experiments
  • Advanced data analysis, coding, kinetic modeling
  • Protein production and functionalization

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