PhD topic in Hormonal Crosstalk in Plant Development Group

6 Jan 2024
Job Information

Organisation/Company

CEITEC, Masaryk University

Research Field

Biological sciences » Biology
Biological sciences » Other
Chemistry » Biochemistry

Researcher Profile

First Stage Researcher (R1)

Country

Czech Republic

Application Deadline

18 Feb 2024 - 23:00 (UTC)

Type of Contract

Temporary

Job Status

Part-time

Offer Starting Date

1 Sep 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Research area:

Regulation of expression of the YUCCA and TAA1 genes, responsible for the production of the plant hormone auxin, during seed development; effects of high temperatures on seed production in Arabidopsis thaliana and Brassica napus

Research group homepage:

https://boisivonlab.ceitec.cz/

Investigating the impact of heat stress on ovule fitness for seed set in Arabidopsis thaliana

Supervisor: Helene Robert Boisivon, Ph.D.

Annotation:

Gametes are key determinants of reproductive success, and their responses to adverse climatic events are particularly relevant for developing heat-resilient plants. Many studies describe the impact of heat stress on pollen development, leading to the production of pollen grain, carrier of the male gametes. Very little is known of the effects of heat on ovules, the female gametophyte. Seed production relies on a successful double fertilization of the egg cell and the central cell by the two sperm cells. In the ovule, a third type of cells, the synergids, attracts the pollen tube to the egg cell for an effective fertilization. The PhD candidate will investigate the effect of heat stress on pollen tube attraction and fertilization of the ovule at molecular and physiological levels, using genetics, microscopy, transcriptomics, and molecular biology approaches.

The Ph.D. topic is part of the collaborative project TANGENC  and will benefit from the COST Action CA22157 RECROP .

Requirements on candidates

The candidate has experience in molecular biology (cloning, RT-qPCR), expression analysis (microscopy), and notions of plant genetics and epigenetics. The candidate will have notions of bioinformatics.

Recommended literature:

Mácová, K; Prabhullachandran, U; Štefková, M; Spyroglou, I; Pěnčík, A; Endlová, L; Novák, O; Robert, H.S. (2022) Long-Term High-Temperature Stress Impacts on Embryo and Seed Development in Brassica napus. FRONTIERS in PLANT SCIENCES 13, 844292. DOI: 10.3389/fpls.2022.844292

Hafidh, S. & Honys, D. (2021) Reproduction Multitasking: The Male Gametophyte. ANNU REV PLANT BIOL 72, 1–34. DOI: 10.1007/s00497-015-0272-4

Völz, R., Harris, W., Hirt, H. & Lee, Y.–H. (2022) ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death. Nat Commun 13, 1746. DOI: 10.1038/s41467-022-29373-7

Keywords: Arabidopsis thaliana, genetics, high temperatures, microscopy, ovules, seed set

APPLY: https://ls-phd.ceitec.cz/how-to-aplly/

Requirements
Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

CEITEC, Masaryk University

Country

Czech Republic

Geofield

Where to apply

Website

https://ls-phd.ceitec.cz/how-to-aplly/

STATUS: EXPIRED