Research Technician III - Virology Lab

The Silva Lab at the University of Pittsburgh is seeking a highly motivated, reliable, and detail-oriented Research Technician to be an integral part of a dynamic research team, conducting independent and collaborative research in a virology-focused laboratory with a diverse and cooperative atmosphere. We study various facets of chikungunya virus, a mosquito-borne virus that causes a severe febrile illness characterized by acute and chronic musculoskeletal manifestations. The position will include performing supervised and independent research at BSL2 and A-BSL3 conditions consisting of 1) tissue culture and virological assays; 2) molecular biology-based assays to uncover mechanisms of viral replication; and 3) pathogenesis studies using murine models of disease.

The incumbent will provide research support to other members of the team and will manage an independent research project, given sufficient training, familiarity, and proficiency. Primary scientific tasks will include conducting complex lab experiments, helping to develop research methods and procedures, maintaining detailed experimental notes, and organizing data and laboratory samples. Responsibilities will include virus stock generation and characterization, cell culture, DNA/RNA extraction, bacterial cloning, RT-qPCR and standard PCR, protein analysis (SDS-PAGE), and basic virological assays. Prior working experience in virological assays is preferred but not required. In addition, the technician will be trained to work in our A-BSL3. Applicants should expect to be comfortable wearing a powered air purifying respirator (PAPR) and other personal protective equipment. The technician will assist with animal colony breeding, maintenance, and animal experiments, which will include infections, tissue harvests, and histological sample preparation in the A-BSL3. Prior working experience with animals is required. Previous work in a BSL3 would be considered highly advantageous, but on-the job training will be provided. Strict adherence to BSL2/BSL3 procedures is essential. The research technician will work closely with the PI and members of the laboratory to ensure that experiments are conducted according to lab protocols.

Other responsibilities for this position include performing common lab duties, such as general lab maintenance, ordering and maintaining lab inventory of supplies and reagents, making common stock solutions, maintaining equipment in working order, and routine duties and maintenance of the A-BSL3 facility. The ideal candidate should have knowledge of basic laboratory skills and must be able to work independently on tasks after training. This position calls for efficiency, creativity, multitasking, and the desire and ability to effectively conduct independent studies in a team setting. The ideal candidate will possess strong organizational skills, efficient time management, scientific curiosity, and analytical problem-solving skills. Excellent communication and interpersonal skills are required, and an open and collaborative personality is highly preferred. Mentoring of undergraduate research workers is anticipated for this position. A Bachelor’s degree in biology or microbiology is preferred, plus 2 years of lab experience with a strong understanding of molecular laboratory techniques and equipment. The position may require some scheduling flexibility.

This position is for the Center for Microbial Pathogenesis (CMP) within the Department of Pediatrics. Equivalent relevant work experience may be substituted for degree requirement. This position is located at UPMC Children’s Hospital of Pittsburgh in Lawrenceville. PA Child Abuse History Clearance, PA State Police Criminal Record Check, and FBI Criminal Record Check will be required prior to the start of employment. Also, a current TB test will be required as a condition of employment. EEO/AA/M/F/Vets/Disabled

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Essential Functions 

•Perform a variety of independent and complex laboratory work and experiments involving cell culture and animal research •Excellent record keeping •Analyze and interpret data, compile results, and prepare reports. •Develop documentation for meetings and publication •Maintain inventory, ordering, and logs • Coordinate and help to manage a small-sized research lab.

Light –Physical effort associated with wet lab work, i.e. standing while conducting experiments, frequent movement to various places, occasionally involving heavy equipment, prolonged standing or sitting, and lifting packages or equipment up to 40 lbs. Applicants must pass fit-test for wearing a respirator (N-95 and PAPR) and should be comfortable with wearing respirator and other personal protective equipment for up to 3 hours. Must possess adequate manual dexterity to perform sensitive laboratory including work with a BSL3/ABSL3 environment.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.