Associate Scientist- Molecular Biology

Pfizer's purpose is to deliver breakthroughs that change patients' lives. Research and Development is at the heart of fulfilling Pfizer's purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best - in - class medicines to patients around the world.

What You Will Achieve

Pfizer's BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory. The group supports non-GMP transient protein lead production across Pfizer's diverse biotherapeutic discovery portfolio to enable Pfizer's research units and their pre-clinical therapeutic in-vitro and in-vivo needs. The BEP DNA production and protein expression group is looking for a associate scientist to provide DNA production and NGS support for the protein expression and purification activities in BEP. The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis. The successful candidate will grow bacterial cultures and perform large-scale DNA production activities following standardized protocols. The candidate will be a key contributor to the scientific team responsible for protein expression and purification.

As an Associate scientist, you will be at the center of our operations and you'll find that everything we do, every day, is in line with an unwavering commitment to quality. Your strong foundation in general scientific practice, its principles and concepts will help in meeting critical deadlines. You will be relied on as a technical contributor to assess the assigned tasks and make decisions that involve direct application of your knowledge.

It is your hard work and dedication that will make Pfizer ready to achieve new milestones and help patients across the globe.

How You Will Achieve It

• Production of plasmid DNA to support transient recombinant protein expression.
  
• Conducting routine molecular biology techniques including cell transformations, day cultures, bacterial cultures, RCA (Rolling Circle Amplification), and digestions.
  
• Maintaining reagent and DNA inventory.
  
• Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations.
  
• Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS).
  
• Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using an online database for inventory management.
  
• Effectively communicating with team members to meet strict schedules and deadlines.
  

Qualifications

Must-Have

• Bachelor's Degree (BA/BS) Biology or applicable Life science degree with 0 to 2 years of relevant work experience.
  
• Excellent communication skills, both verbal and written .
  
• Experience with data analysis and presentation software.
  

Nice-to-Have

• Understanding of DNA purification and NGS.
  
• Understanding of routine molecular biology techniques.
  
• Experience or knowledge in Python or PyTorch for automation.
  
• Experience in design and development of a novel SLA-print 3D printing!
  

PHYSICAL/MENTAL REQUIREMENTS

Other Job Details

• Work Location Assignment:On Premise
  

The annual base salary for this position ranges from $54,700.00 to $91,100.00. In addition, this position is eligible for participation in Pfizer's Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life's moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage. Learn more at Pfizer Candidate Site - U.S. Benefits | (uscandidates.mypfizerbenefits.com). Pfizer compensation structures and benefit packages are aligned based on the location of hire. The United States salary range provided does not apply to Tampa, FL or any location outside of the United States.

Relocation assistance may be available based on business needs and/or eligibility.

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development