Research Engineer at the division of Biochemistry and Structural Biology at the department of chemistry KILU (PA2023/4041)

Lund University was founded in 1666 and is repeatedly ranked among the world’s top universities. The University has around 45 000 students and more than 8 000 staff based in Lund, Helsingborg and Malmö. We are united in our efforts to understand, explain and improve our world and the human condition.

Lund University welcomes applicants with diverse backgrounds and experiences. We regard gender equality and diversity as a strength and an asset.

The work duties include, but are not limited to

• Imaging of pure amyloid fibrils and amyloid co-aggregates with chaperones or membranes by cryo-TEM, including freezing of samples on grids and operating the cryo-TEM.
• Structure determination of amyloid fibril and amyloid co-aggregates with chaperones or membranes using cryo-EM.
• Imaging of amyloid fibril and amyloid co-aggregates with chaperones or membranes by super-resolution fluorescence microscopy
• Protein-protein interaction analysis using SPR technology.
• Protein expression in bacterial cells.
• Protein purification using ion exchange chromatography and size-exclusion chromatography.
• Protein labeling with fluorophores.
• Kinetics and equilibrium studies of protein amyloid formation.

Education: Ph D in Biochemistry, Biophysical Chemistry, Physical Chemistry or equivalent. Documented hands-on experience of cryo-TEM imaging, including freezing of samples on grids using Vitrobot and Leica plunge freezer and operating the cryo-TEM models such as Jeol JEM 2200FS with side entry sample loader and Titan Krios.

• Documented hands-on experience of structure determination of in-vitro and ex-vivo amyloid fibrils of several different proteins using cryo-EM including data acquisition with SerialEM and EPU and data processing using Relion and cryoSpark.
• Documented hands-on experience of protein expression and purification from bacterial expression systems using ion exchange chromatography and size-exclusion chromatography.
• Documented hands-on experience and expertise in of protein amyloid formation and global kinetic analysis to derive the underlying mechanism.

Documented hands-on experience and expertise of amyloid formation mechanism studies using super-resolution fluorescence microscopy. Documented experience of purification of amyloid fibrils from tissue samples Documented hands-on experience of automated chromatography systems such as FPLC and HPLC.

• Excellent proficiency in English, spoken and written.

We will place great emphasis on personal suitability. Consideration will also be given to good collaborative skills, drive and independence, and how the applicant’s experience and skills complement and strengthen the scientific work within the department, as well as contribute to its future development.

Fixed-term employment for 10 months, full time (20%).

Preferred starting date: March 1, 2024.

Contact person: Sara Linse.

Applications should include a cover letter stating the reasons why you are interested in the position and in what way the employment corresponds to your qualifications. The application should also contain a CV with list of publication, degree certificate or equivalent, and other documents you wish to be considered (grade transcripts, contact information for your references, letters of recommendation, etc.).