Post-doctoral position

We have a fellowship funded by the ANR for a post-doctoral fellow specialized in microbiome studies at the Institut Pasteur. The selected candidate will be working at the Biology and Genetics of the Bacterial Cell Wall Unit headed by Ivo G. Boneca. This is a collaborative project with the teams of Raquel Mejias-Luque of the Technical University of Munich and of Richard Ferrero of the Monash University.

The project builds on our previous work on the dissemination of peptidoglycan fragments across the body from the gut. We are looking for somebody that has highly transversal expertise in biochemistry of peptidoglycan, mass spectrometry, cellular and animal models, and in confocal/immunofluorescent microscopy. The postdoc will study the model organism Helicobacter pylori and its mechanisms of release of immune-stimulatory peptidoglycan fragments to shape the host immune system using in vitro organoids, gut-on-chip and in vivo models.

The successful candidate should have a strong expertise in microbiology and organoids. Expertise with animal models and/or by LC-MSMS analysis would be a plus.

Viala J, C Chaput, IG Boneca, A Cardona, SE Girardin, AP Moran, R Athman, S Mémet, M Huerre, AJ Coyle, PS DiStefano, PJ Sansonetti, A Labigne, J Bertin, DJ Philpott, RL Ferrero. 2004. Nod1 responds to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island. Nat Immunol. 5(11):1166-1174. doi: 10.1038/ni1131.

Grubman A, M Kaparakis, J Viala, C Allison, L Badea, A Karrar, IG Boneca, L Le Bourhis, S Reeve, IA Smith, EL Hartland, DJ Philpott, RL Ferrero. 2010. The innate immune molecule, NOD1, regulates direct killing of Helicobacter pylori by antimicrobial peptides. Cell Microbiol. 12(5):626-639. doi: 10.1111/j.1462-5822.2009.01421.x.

Irving A, H Mimuro, T Kufer, C Lo, R Wheeler, L Turner, BJ Thomas, C Malosse, MP Gantier, LN Casillas, BJ Votta, J Bertin, IG Boneca, C Sasakaw, DJ Philpott, RL Ferrero, M Kaparakis-Liaskos. 2014. NOD1 interacts with peptidoglycan and RIP2 on early endosomes to promote RIP2-dependent autophagy and inflammatory signaling. Cell Host Microbe. 15(5):623-635. doi: 10.1016/j.chom.2014.04.001.

Dias Bastos PA, R Wheeler, IG Boneca. 2021. Uptake, recognition and responses to peptidoglycan in the mammalian host. FEMSMicrobiol Rev. 45(1):fuaa044. https://doi.org/10.1093/femsre/fuaa044

Wheeler R, PA Dias Bastos, O Disson, A Rifflet,I Galbanyi,J Spielbauer, M Berard, M Lecuit, IG Boneca. 2022. Microbiota-induced active translocation of peptidoglycan across the intestinal barrier dictates its within-host dissemination. Proc Natl Acad Sci U S A. 120(4):e2209936120. doi: 10.1073/pnas.2209936120 .

Applications (a motivation letter, a full CV and minimum of two references) should be sent to Ivo G Boneca ([email protected] ).