Research logistician/Engineer position (with PhD) in flow cytometry

3 Mar 2024
Job Information

Organisation/Company

UNamur (Université de Namur)

Research Field

Biological sciences

Researcher Profile

Recognised Researcher (R2)
Leading Researcher (R4)

Country

Belgium

Application Deadline

26 Apr 2024 - 23:00 (UTC)

Type of Contract

To be defined

Job Status

Full-time

Hours Per Week

To be defined

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Position

Full-time (100%) research logistician/engineer (fixed-term) in flow cytometry for the flow cytometry core facility of UNamur (Belgium)

 

Work environment

The Morph-Im (Morphology & Imaging) core facility (https://platforms.unamur.be/morph-im ) is one of the nine technological platforms of Université de Namur and is dedicated to offer the state-of-the art in imaging technologies, both for life science and surface material applications. The platform gathers a wide variety of state-of-the-art instruments and expertise, such as electron and optical microscopy, atomic force microscopy, scanning tunneling microscopy, histology and flow cytometry. Morph-Im includes now 3 flow cytometers (2 analysers and 1 cell sorter).

 

Your missions

• Management of the users, computers and reagents

• Daily and annual maintenance

• Contacts with the flow cytometer companies

• Support to researchers/private companies for the culturing, sterile and non-sterile sorting and data analyses for punctual needs

• Training of researchers for the culturing, sterile and non-sterile sorting and data analyses for recurrent needs.

• Project Preparation: assistance with labelling (dissociation, fixation, blocking, permeabilization, dilution of antibodies…), discussions with the researchers/customers about their model, their research question, and controls, assistance in the theoretical design of complex multicolor panels (tandems, brightness, co-expression…).

• Analysis of samples with multiple labelling (gating strategy…).

• Technology monitoring (software, fluorochromes, sample preparation).

• You will be encouraged to seek funding from public agencies or private partners and provide flow cytometry-based solutions to external clients.

• Pipelines setup, guide design, collaboration with the user.

• You will be asked to develop a genome-edition core facility for mammalian cells by establishing adapted CrispR-Cas protocols combined with cell sorting in the frame of research projects conducted at UNamur and possibly in private companies.

 

Skills/Qualifications

1)   Diplomas

You hold:

• an engineer degree or a master degree in science or another relevant discipline,

• AND a doctoral thesis during which you developed strong expertise in flow cytometry and ideally in genome editing.

• N.B. : Candidates who have not yet obtained the academic degree of Doctor can apply only if they are about to defend their doctoral thesis (at the latest within 3 months following the appointment).

2)   Professional experience

At least 2-year professional experience in flow cytometry.

3)   Required Skills

You have the following skills:

• Excellent theoretical and practical knowledge of flow cytometery (analyzer and sorters), with expertise in designing complex flow cytometry-based experiments. The expertise of the candidate should be demonstrated by publications in peer-reviewed journals.

• Mastery of labelling techniques for (1) studying eukaryotic and bacterial cellular processes such as the cell cycle (G1, S, G2-M), ploidy, cell viability, and apoptosis, and (2) performing phenotyping (immune cells, organoids, intracellular signaling, mitochondrial potential…).

• Good experience with FlowJo software is a plus (training users on FlowJo).

• Multitasking abilities, excellent sense of priorities and organization, autonomy. You will have to manage several projects simultaneously: support in flow cytometry (analysis and cell sorting) for several facility users/projects.

• Strong team spirit: you will work with other team members.

• Strong service spirit: you must appreciate to collaborate on the projects of other researchers.

• Excellent communication abilities: you must be proficient in English (oral and written).

Knowledge and skills in bold will be assessed during the recruitment process.

4)   Advantages

• Good practice of cell biology tools (cell culture, immunofluorescence…).

• Good practice of biochemistry/molecular biology tools (cloning, DNA and protein electrophoresis, Western blotting…).

• Practical knowledge/experience of CRISPR technology using transfection/transduction. The experience of the candidate should be demonstrated by publications in peer-reviewed journals.

 

Contract: one-year fixed-term contract (100%)

 

Starting date: as soon as possible

 

How to apply

The application file, consisting of a detailed curriculum vitae, a cover letter and the completed application form, must be sent for April 26st at the latest. For more details, see Application URL.

Requirements
Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

UNamur (Université de Namur)

Country

Belgium

Geofield

Where to apply

Website

https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-e…

Contact

Website

http://www.unamur.be

STATUS: EXPIRED