Laboratory assistant microbial fermentation and purification

MITH is an academic research group developing diagnostic and therapeutic biopharmaceuticals with a strong vision to bring innovation from the bench to bedside. We have established a cleanroom facility for the production of lead biomolecules for human clinical trials. With this unit we want to valorize our laboratory studies by bringing them into a GMP environment and thus guarantee safe testing on humans at the UZ Brussels university hospital. We are looking for a motivated professional with a passion for pharmaceutical quality processes.

Techniques: microbial manipulation (P. pastoris), cell banking, fermentation in a small-scale (30L) bioreactor, cell collection, cell extraction, protein purification via chromatography, filtration, precipitation device management, validation of fermentation processes and devices, working in a clean room environment, writing procedures and protocols.

Here is a selection of your tasks:

• Carrying out operational activities in a GMP clean room facility.
• Taking responsibility for the fermentation branch of the GMP unit.
• Setting up procedures to ferment microbes and extract proteins.
• Setting up procedures to purify proteins from a microbial extract.
• Scaling up protein purification procedures to liter-level.
• Evaluating the robustness of purification procedures.
• You write standard operating procedures in English.
• You contribute to the continuous improvement and maintenance of the quality management system.
• You ensure the qualification and maintenance of the equipment and infrastructure related to the fermentation procedures.
• Taking responsibility for the proper documentation of the performed processes.
• As a team you take up the challenge to deliver proteins for clinical studies.
• You are open to continuous training and self-study.

For this position, the Brussels Health Campus in Jette will be your home base.