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. References: https://doi.org/10.1002/rcm.7475; https://doi.org/10.1002/bit.27661 Keywords Liquid Chromatography; Mass Spectrometry; Metabolomics; Biomanufacturing; Bioinformatics Eligibility citizenship Open
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protein expression in Escherichia coli and mammalian cell lines through bacterial culture, transient transfection, and stable cell line generation. Purify proteins using affinity chromatography, ion
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-exclusion, and ion-exchange chromatography as appropriate. • Characterize proteins, antigens, and protein nanoparticles using SDS-PAGE, HPLC, biolayer interferometry (BLI/Octet), dynamic light scattering
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for optimal protein expression. Purify protein from bacteria by immobilized metal affinity chromatography, size exclusion chromatography, and ion exchange chromatography. Develop assays to measure the
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(SEC), and ion exchange chromatography on FPLC/ÄKTA platforms Perform rigorous quality characterization including SDS-PAGE, western blot, ELISA, dynamic light scattering, and binding validation by SPR
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Universitat Politècnica de Catalunya (UPC)- BarcelonaTECH | Barcelona, Cataluna | Spain | 14 days ago
Technical Skills: Ammonia analysis. Use and handling of ion chromatography for sample analysis. MatLab. Specific Requirements Knowledge: Modeling of membrane processes. VMD (vacuum membrane distillation
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Universitat Politècnica de Catalunya (UPC)- BarcelonaTECH | Barcelona, Cataluna | Spain | about 10 hours ago
pilot installations developed externally at the UPC. Where to apply Website https://seuelectronica.upc.edu/en/procedures/call-for-recruitment-of-ptgas-staf… Requirements Research Field Chemistry
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transcription-translation) expression systems. Practical experience with protein purification by FPLC (IMAC, ion exchange, size-exclusion chromatography). Experience with protein characterisation and QC
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, sediments/soils, sludges, biota, etc.), including sample extraction, extract purification, chromatography, and mass spectrometry. Experience with QA/QC procedures. Experience in collecting
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such as biological quantification, PCR, ion chromatography, spectrophotometry and solid analysis Statistical data analysis Designing new experimental bench-scale set ups Working with pilot plants You