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with non-mammalian model organisms (e.g., Drosophila, C. elegans, Xenopus, or zebrafish); experience with fluorescence microscopy, as well as digital image acquisition and analysis; experience with
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techniques in cell culture (routine cell maintenance, transient transfections, preparation of nuclear extracts, utilization of CRISPR/Cas9) Assist with fluorescent imaging techniques, both biochemical and in
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Design AAV vectors for gene therapy of mouse models Capture fluorescent images using microscopy and quantify data Purify proteins and perform western analyses Maintain a mouse colony and establish genetic
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fluorescent labels. Raman micro-spectroscopy, primarily using 785 nm excitation, will obtain molecular fingerprints associated with lipids, proteins, nucleic acids and metabolites. Complementary UV–visible
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, electrophysiology, and live-cell imaging Apply and further develop methods for high-resolution and super-resolution fluorescence microscopy Analyze in vivo models, particularly using slice electrophysiology, protein
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. Proficiency in fluorescence microscopy imaging techniques is also required. Skills in processing and quantifying scientific images (e.g., using ImageJ/Fiji or equivalent software) are additionally desirable
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Supervisor: Dr Guiping Wang Course start date: 1st October 2027 Project details For further information about the research group, please visit our website at https://www.cruk.cam.ac.uk/research
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Previous research experience with C. elegans is highly desirable but not required. Experience with genetics, molecular biology, PCR/genotyping, cloning, fluorescence microscopy, or image analysis is a plus
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; running live-cell imaging experiments that capture chromosome segregation errors as they happen; performing fluorescence in situ hybridization (FISH) and molecular assays to measure aneuploidy and copy
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 16 days ago
: * Advanced fluorescence microscopy and image analysis * Cell biology and organelle biology * Molecular biology and cloning * Bioinformatics or computational analysis experience * Experience working with large