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. Performing biochemical and enzymatic assays focused on protein-protein interactions, enzyme and protein kinase activity and kinetics, and signalling pathways associated with cancer biology. Collaboration with
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(RNA/protein extraction, RT-PCR, enzyme-linked immune-sorbent assay (ELISA), western blotting). Background in immunology, particularly innate immunity. Knowledge, Skills and Abilities: Excellent
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encodes the lysosomal enzyme glucocerebrosidase. The lysosome is an intracellular organelle responsible for the degradation of toxic substances, and approximately 50% of patients with Parkinson’s disease
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basic unit of biology. Topics include structure and roles of biologically important molecules, prokaryote and eukaryote cell structure, concepts of metabolism and energy flow, enzymes, photosynthesis, ATP
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genome architecture is DNA methylation, which is regulated, among others, by TET enzymes. Altered TET1 activity has been observed in many cancers, but its consequences remain poorly understood. Our
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. The Biodemography Lab primarily uses enzyme immunoassays to measure biochemical markers and qPCR-based assays to measure molecular markers in a variety of biospecimen types, often for large-scale population health
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Duke University - Biochemistry & Cell Biology | Durham, North Carolina | United States | about 2 months ago
biochemistry, drug design, immunobiology, neuroscience, and cancer biology. Example application areas include generative models for protein/enzyme design, drug discovery, foundation models of neural activity and
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notebook on plasma and tissue specimens: Enzyme-Linked Immunosorbent Assay (ELISA); optimize and perform both kits and assays de novo depending on the availability of commercial kits for target antigens (i.e
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; experience and expertise in the field - experience in the cloning, isolation, purification, structural and functional characterization of recombinant proteins/enzymes good knowledge (reading, speaking
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; experience and expertise in the field - experience in the, isolation, purification, immobilization of recombinant proteins/enzymes, monitorization of enzymatic reactions good knowledge (reading, speaking