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Field
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cytometry and cell sorting is also required together with experience of advanced genome editing including cloning of CRISPR gRNAs for custom screens, base editing (including alternative specificity Cas
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. Generate genetically modified cell lines using CRISPR/Cas9 genome editing. Produce lentiviral vectors and perform lentiviral transductions. Design and construct plasmids using standard molecular cloning
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-domain proteins. Research program The project will establish a targeted CRISPR-based functional screening strategy for LCCL-domain proteins in Cryptosporidium. The postdoctoral researcher will
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. The project will use quantitative mitochondrial-tracing approaches, including a recently established HiBiT-based platform, live-cell imaging, CRISPR-engineered cancer models and tumour–stromal co-culture
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ambitious research project that bridges translational and clinical science? In our lab, your work directly contributes to science that makes a difference. The Salzer Group combines CRISPR engineering of
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research. Familiarity with single-cell or spatial omics, CRISPR screening, or advanced genomic technologies. Experience working in internationally competitive research environments. Clear potential
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with experience of advanced genome editing including cloning of CRISPR gRNAs for custom screens, base editing (including alternative specificity Cas enzymes) and prime editing. Experience of off-target assays
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strong background in cellular engineering and gene editing (including CRISPR-Cas9 and CRISPR-based screens) and demonstrated experience culturing human stem cells (iPSCs). You will have excellent data
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-throughput CRISPR-based functional genomics approaches, we systematically identify genes and pathways that enable tumor cells to evade immune attack, providing new targets for therapeutic intervention and
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production. CRISPR gene editing will also be used to correct the mutations in patient cells and determine whether normal cellular function can be restored. This research will provide new insights