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metabolic dysfunction, DNA damage, and cancer progression. · Utilize mouse models and CRISPR gene editing. · Design, execute, and optimize molecular, cellular, and animal experiments with
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• Experience with immune functional assays, multi-parameter flow cytometry, and genetic engineering approaches (e.g., CRISPR/Cas9) • Strong data analysis skills and ability to interpret mechanistic findings
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purification of recombinant proteins and the functional analysis of proteins is desired. Experience in cell biology, including CRISPR/Cas9 approaches, transient transfections, and PCR is also preferred
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. We are seeking a Research Fellow to join a new research group using CRISPR functional genomics and stem-cell-derived organoids to explore human brain development and evolution. This is a rare
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Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S) | Portugal | 2 months ago
display of well-defined ErbB2 glycoforms, through the CRISPR/Cas9-based precise glycogene editing of two ERBB2-amplified GC cell lines. Produced ErbB2 glycoforms will be structurally elucidated by mass
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, protein biochemistry and/or cancer biology. Experience with CRISPR genome editing, mouse models or omics technologies is considered an advantage. Contact(s) for information on the position (identity
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Responsibilities: Develop and optimize strategies for multiplex CRISPR-Cas genome editing in Brassica Design, construct, and validate CRISPR-Cas vectors for efficient gene editing Generate edited Brassica plants
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(FVII) deficiency, a rare but serious bleeding disorder caused by mutations in the F7 gene, leading to markedly reduced FVII activity, and applies advanced technologies such as CRISPR-based gene editing
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experiments using tools such as cell imaging, CRISPR, chemoproteomics, protein purification or structural biology are required. Desired Qualifications* Programming skills in Python or R are desired. Modes
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to learn to: Perform animal studies: mouse and large animal inoculation, tissue sampling, necropsy techniques Conduct molecular biology: genomic DNA extraction, PCR, gene cloning, CRISPR/Cas genome editing