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. These pathogens use the surface proteins IcsA and ActA to recruit host actin-regulatory factors and generate actin comet tails that propel the bacteria through infected cells. The successful candidate will combine
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with disease induction (EAU), adoptive transfers, and genetic fate mapping. DNA/RNA extraction, PCR/qRT-PCR, Western blot, mAb purification, comet assays, and enzyme kinetics/spectroscopy. Sterile
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comet tails that propel the bacteria through infected cells. The successful candidate will combine biochemical reconstitution, fluorescence microscopy and structural biology to determine how this process
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) and genotoxicity assessment (comet assay); Experience with core molecular/cell biology techniques such as immunofluorescence, immunoblotting, flow cytometry, quantitative PCR, and confocal microscopy