Research assistant

In the Brodin lab, the team performs state-of-the-art immunomonitoring analyses. The lab receives samples, mostly from blood, and prepares these using a unique liquid handling robotic platform, stain cells with antibodies and acquire data by high-dimensional spectral cytometry, single-cell mRNA-sequencing, and plasma protein analyses.

The lab is looking to hire a research assistant to be responsible for handling and processing blood samples for liquid handling robotics and miniaturized cultures of blood cells. The work is hands-on in the laboratory and involves sample preparation, cell cultures, reagent preparations, performing stimulation experiments, staining and acquisition of samples by spectral cytometry. This position is full time employment, 40h week for 6 months.

The Petter Brodin research group develops and applies experimental and computational research methods for systems-level analyses of human immune systems. The aim of the team is to understand human immune system variation in health and disease and the development of immune systems early in life. The group is half experimental and half computational and belongs to the unit of clinical pediatrics, which is a broad, multi-disciplinary unit at the Department of Women’s and Children’s Health, KI.

The responsibilities of this candidate in the lab will mainly involve:

• Clinical sample reception (both onsite and offsite) and cryosample management.
• Staining and acquisition of samples by multicolor spectral cytometry.
• Processing and recording the sample/subject metadata in a database by coordinating with the responsible experimentalist.
• Large-scale planning and handling of clinical samples (hundreds of clinical samples each day) and their organization into plates for proteomic analysis.
• PBMC prep, plasma prep, and cell isolation methods.
• Liquid handling robotics usage and miniaturized cell cultures.

The research assistant will be an integral member of the Brodin research lab and will participate in many different ongoing research projects and will be able to learn many cutting edge technologies used in the facility and the research group (https://www.brodinlab.com/).

The successful candidate should have extensive lab experience particularly with methods like blood sample handling, cultures, reagent/buffer preparation, and experience with spectral cytometry. Prior experience in record maintenance (with basic computer skills) of clinical samples and reagents will be helpful. Demonstrated experience in spectral cytometry and a grasp of new technological development and its understanding will be a plus. The successful candidate must thrive in interdisciplinary environments and should be dedicated to performing the tasks assigned in the lab meticulously and with great care. The candidate must be comfortable working both independently and in collaboration with the lab heads and other members of the team (when necessary) and contribute to the team’s concerted efforts. The candidate must have good communication skills in English as the team is multicultural and interdisciplinary.

A selection will be made among qualified applicants based on the following criteria:

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Solna, Biomedicum

https://brodinlab.com/

https://staff.ki.se/people/petter-brodin

Welcome to apply at the latest May 30 2024.

The application is to be submitted through the Varbi recruitment system. In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form.

Permanent position is initiated by a six months trial period.