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), primary cell culture from patient bone marrow/peripheral blood samples, PBMC isolation (density gradient centrifugation, e.g., Ficoll-Paque), and Bone marrow mononuclear cell isolation and processing
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adult offspring immune organs, followed by sample processing and downstream analyses. Perform mammalian cell culture, transfection, CRISPR-Cas9 gene editing and clone validation, flow cytometry, immune
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and developmental outcomes. Utilize locus-specific and next-generation molecular and genomic techniques, including RNA-seq, RRBS, WGBS, EPIC arrays, and related methodologies in human and mouse samples
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scientific reports. Bank and catalog biological samples; maintain research databases and project inventories; work independently in conducting research activities; assist in training and mentoring research