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techniques. Perform laboratory work in recombinant protein expression in different platforms such as E. coli, yeast, protein analysis such as SDS-PAGE, Immunological assays such as ELISA, Western blot
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maintaining an evolution experiment with E. coli, requiring daily maintenance. Our lab studies evolutionary and ecological processes by conducting evolution experiments with bacteria. Duties include (i
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macrophages, neutrophils and T cells Cytokine biology and immune signaling pathways (e.g. G-protein signaling) Pathogens of interest include E. coli , S. pneumoniae , Legionella spp., and others. Projects will
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include expressing recombinant proteins in insect, mammalian, and E. coli cells; purifying proteins using FPLC systems; performing in vitro RNA transcription and labeling; carrying out in vitro assays
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preparation, and standard E. coli genetic manipulations; carry out DNA and RNA extraction, quality assessment, and handling under RNase-free conditions; prepare RNA-seq and DNA sequencing libraries for both
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/ProvinceElvasCityVairãoPostal Code4485-655 StreetRua dos Lagidos - Lugar da Madalena Contact State/Province Lisboa City Oeiras Website https://www.iniav.pt Street Quinta do Marquês, Av. da República Postal Code 2784505 E-Mail
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preparation, and standard E. coli genetic manipulations; carry out DNA and RNA extraction, quality assessment, and handling under RNase-free conditions; prepare RNA-seq and DNA sequencing libraries for both
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isolation/sequencing, cloning) across multiple organisms (E. coli, C. elegans, S. cerevisiae, and M. musculus). Lead protein expression, purification, and post-translational modification efforts. Identify and
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chemical materials and infectious diseases are present. Specific hazards of the position are, but are not limited to salmonella, Listeria, E. coli, Campylobacter, hot acids, bases, ethers and other chemicals
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, chemistry, biology, etc.) Knowledge and experience in molecular biology The following skills would be an advantage: establishing bioassays, cloning, protein expression, working with cell cultures, E. coli and