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USDA-ARS High-Throughput In Vitro Screening of Antimicrobial Compounds Against Phytoplasma in Cherry
will gain hands-on experience utilizing an in vitroplant tissue culture and molecular screening platform to evaluate candidate therapeutic compounds (such as EPA-registered antimicrobials, antimicrobial
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communities, testing dietary and other control modalities, as part of the learning experience. This will include various culture methods, nucleic acid extraction, PCR methods, sequence analysis
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of Defense . Qualifications The ideal candidate will be a graduate student or received a master's or doctoral degree in Civil or Environmental Engineering, or Hydroligic Sciences. Preferred skills include
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with these plants using in vitro and in vivo animal models. Animal tissue and cell culture models will be used to study the ability of compounds of interest to cross absorptive barriers as
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and researchers engaging in organoid/cell biology research activities including, but not limited to, nutritional physiology, immune system modulation, and gastrointestinal cell culture to support gut
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analyses for methods such as flow cytometry, cell culture/stimulations, and RNA sequencing (bulk, single-cell, spatial) may also be available based on your interests. Research endeavors are intrinsically
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opportunities to interpret and communicate research findings. Exposure to additional assays and analyses for methods such as flow cytometry, cell culture/stimulations, and RNA sequencing (bulk, single-cell
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, real time PCR, protein expression, tissue culture, construction of recombinant virus, Western blotting, light and fluorescence microscopy, and flow cytometry. You may also have opportunities to use our
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your skills in molecular biology, virology, cell culture, vector biology, and animal models, to understand what drives the arbovirus transmission cycle. Additionally, you will be provided opportunities
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platforms (bioreactors/biomaterials), high content imaging, and extracellular vesicle characterization. Specific learning objectives include: Gaining hands-on experience with advanced cell culture techniques