PhD position in Biotechnology

Updated: 12 days ago
Job Type: FullTime
Deadline: 31 May 2024

17 Apr 2024
Job Information
Organisation/Company

Slovak University of Agriculture in Nitra
Department

Faculty of Biotechnology and Food Science
Research Field

Agricultural sciences
Researcher Profile

First Stage Researcher (R1)
Country

Slovakia
Application Deadline

31 May 2024 - 23:59 (Europe/Bratislava)
Type of Contract

Not Applicable
Job Status

Full-time
Hours Per Week

37,5
Offer Starting Date

1 Sep 2024
Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme
Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Heavy metal detoxification mechanisms in lichens with a focus on use in soil phytoremediation.

 

Supervisor: prof. RNDr. Martin Bačkor, DrSc.

Workplace: Faculty of Biotechnology and Food Science, Slovak University of Agruculture in Nitra

Field of Study: Biotechnology

Study program: Agrobiotechnology

Form of study: full-time/part-time

Language: Slovak

Abstract

The main goal of the proposed topic of the dissertation is the study of the phytoremediation potential of lichens, connected with the understanding of species-specific mechanisms of heavy metal toxicity, or tolerance towards them. Because of the advantages of symbiosis, lichens can grow in habitats where none of the symbionts can survive as an independent organism. Lichens can accumulate heavy metals in amounts that exceed their nutritional requirements, up to 10% of their dry weight. Within the implementation of the topic, changes in the composition of low molecular weight compounds, including cysteine, glutathione and phytochelatins, will be studied, as well as changes in the content of ascorbic acid, which is considered one of the most important antioxidants in cells. Changes in the composition of acids that are part of the Krebs cycle and the production of reactive forms of oxygen and nitrogen will also be analyzed. The expression of selected proteins responding to an excess of heavy metals in the environment (eg heat shock proteins) will be analyzed using Western blotting and RT-PCR techniques.

Mechanizmy detoxikácie ťažkých kovov v lišajníkoch so zameraním na využitie vo fytoremediácii pôd.

Abstrakt

Hlavným cieľom navrhnutej témy dizertačnej práce je štúdium fytoremediačného potenciálu lišajníkov, spojeného s pochopením druhovo špecifických mechanizmov toxicity ťažkých kovov, resp. tolerancie voči nim. Kvôli výhodám symbiózy, lišajníky môžu rásť v biotopoch, kde žiadny zo symbiontov nemôže prežiť ako samostatný organizmus. Lišajníky dokážu akumulovať ťažké kovy v množstvách, ktoré prevyšujú ich nutričné požiadavky, až do 10% ich suchej hmotnosti. V rámci realizácie témy sa budú študovať zmeny v zložení zlúčenín s nízkou molekulovou hmotnosťou, vrátane cysteínu, glutatiónu a fytochelatínov, ako aj zmeny obsahu kyseliny askorbovej, ktorá sa považuje za jeden z najdôležitejších antioxidantov v bunkách. Budú sa analyzovať aj zmeny v zložení kyselín, ktoré sú súčasťou Krebsovho cyklu a produkcii reaktívnych foriem kyslíka a dusíka. Expresia vybraných proteínov reagujúcich na nadbytok ťažkých kovov v prostredí (napr. proteíny tepelného šoku) sa bude analyzovať s využitím techník Western blotting a RT-PCR.

 

More information about PhD position

Additional research info:[email protected]

https://fbp.uniag.sk/sk/agrobiotechnol%C3%B3gie-6624/

https://fbp.uniag.sk/sk/phd-stupen/

https://is.uniag.sk/prihlaska/

 


Requirements
Research Field
Agricultural sciences
Education Level
Master Degree or equivalent

Languages
SLOVAK
Level
Good

Additional Information
Work Location(s)
Number of offers available
1
Company/Institute
Faculty of Biotechnology and Food Science, Slovak University of Agruculture in Nitra
Country
Slovakia
City
Nitra
Postal Code
94976
Street
Tr. A. Hlinku 2
Geofield


Where to apply
Website

https://is.uniag.sk/prihlaska/

Contact
State/Province

Slovakia
City

Nitra
Website

http://www.uniag.sk/sk/
Street

Trieda Andreja Hlinku 2
Postal Code

949 76
E-Mail

[email protected]

STATUS: EXPIRED

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