Recruitment for the Poznań Doctoral School of the Institutes of the Polish Academy of Sciences at...
Recruitment for the Poznań Doctoral School of the Institutes of the Polish Academy of Sciences at the Institute of Plant Genetics, PAS in Poznań
Procedure no. 20/2021/IGR/PSD
INSTITUTION: Institute of Plant Genetics, PAS
CITY: Poznan
POSITION: Ph.D. student
POSITIONS AVAILABLE: 1
SCIENTIFIC DISCIPLINE: agriculture and horticulture
PUBLICATION DATE: Jul. 27, 2021
APPLICATION DEADLINE: Aug. 31, 2021
WWW: http://www.igr.poznan.pl/en/main-en/ids-en/competitions
IGR PAN:http://www.igr.poznan.pl/en/home-en
PSD IPAN:http://www.psd-ipan.ibch.poznan.pl/index-en.html
KEY WORDS: Calvin cycle, chlorophyll fluorescence, de novo sequencing and assembly of F. glaucescens genome, enzymatic antioxidant system, gas echange, lipidome, monocot species transformation, plant genome editing using CRISPR/Cas9 technology, RWC
Research topic: The main goal of the project is characterization of lipocalins family and recognition of chloroplastic lipocalins function in protection of photosynthetic apparatus against oxidative stress in Festuca glaucescens. The detailed goals are as follows: (i) identification of the genes encoding lipocalins (including true lipocalin and lipocalin-like proteins) within F. glaucescens genome, (ii) cellular function analysis of true lipocalins in the wild-type plants, (iii) generation of transgenic plants with CHL gene/genes knockout, (iv) analysis of photosynthesis activity in the wild-type plants and CHL/knock-out transgenic plants exposed to combined drought and high light treatment, (v) analysis of thylakoid membrane lipidome in the wild-type plants and CHL gene/genes knock-out transgenic plants exposed to combined drought and high light treatment.
Principal Investigator: dr. Izabela Pawłowicz – the leader of the whole project, implemented in the consortium IPG PAS – IP PAS – NIL and the PI of the part of the project carried out at IGR PAN
DESCRIPTION:
Place of employment: Plant Physiology Team, Institute of Plant Genetics of the Polish Academy of Sciences
Supervisor: dr. Izabela Pawłowicz
Goal of employment: implementation of the OPUS 20, no. 2020/39/B/NZ9/02488
Scope of research: Annotation of the genes encoding TIL and CHL lipocalins, identification of the ligands that bind to TIL and CHL lipocalins, generation of F. glaucescens transgenic plants with CHL gene/genes knock-out (gene editing with CRISPR/Cas9 use), leaf physiological parameters measurements including: relative water content (RWC); lipid peroxidation (TBARS assay); reactive oxygen species (ROS) content and abscisic acid (ABA) content in the wild-type plants and knock-out mutants in the control conditions and under simultaneous drought and high light treatment, functioning of antioxidant system analysis including: expression and activity of catalases, peroxidases and dismutases measurement in the wild-type plants and knock-out mutants in the control conditions and under simultaneous drought and high light treatment, functioning of Calvin cycle analysis including: chlorophyll fluorescence and gas exchange measurement; expression and activity of pFBA in the wild-type plants and knock-out mutants in the control conditions and under simultaneous drought and high light treatment, lipidome analysis of thylakoid membrane in the wild-type plants and knock-out mutants in the wild-type plants and knock-out mutants in the control conditions and under simultaneous drought and high light treatment.
Duties in project: Generation of transgenic plants; physiological measurements, gene expression profiling at transcript and protein (RT-qPCR and western blot), contribution in manuscript preparation. Analysis and interpretation of results. Preparation of publications and other forms of presentation of results.
Requirements for the candidates:
10. Additional scientific activity (publications, conference announcements and other forms of presenting results, participation in projects, research clubs, etc.) and organizational activity (eg organization of workshops, trainings, conferences) is welcome.
11. Mobility is welcome: internships, workshops, training, etc.
Additional information:
1. Research and doctoral theses shall be carried out within the OPUS 20, no. 2020/39/B/NZ9/02488, entitled “Characteristics of lipocalin family and their functioning in photosynthetic apparatus stabilizing under oxidative stress in Festuca glaucescens”, funded by National Centre of Science.
2. PhD students shall receive a stipend in the gross amount of ca. 4270,50 PLN (3685,00 PLN net), for the period of 48 months.
3. PhD students shall be subject to social insurance, pursuant to article. 6 section 1 passage 7b of the act of October 13th, 1998 on the social insurance system (Journal of Laws of 2019, item 300, 303 and 730).
Required documents:
ATTENTION: at the stage of the recruitment process, there is no requirement to present documents certified by the apostille clause nor the requirement of nostrification of diplomas. These requirements must be met if the candidate is accepted.
Documents in the electronic form (in 1 pdf file) must be sent by e-mail to: to [email protected] putting in the title:
Submission deadline is 31 August 2021.
Criteria for evaluation of candidates:
The description of the recruitment process is stipulated in the Regulations of Recruitment for PDS IPAS. Following the recruitment procedure, the unadmitted candidates shall be informed on the number of points obtained at both stages.
For additional information please contact the Principal Investigator:
Dr. Izabela Pawłowicz
e-mail: [email protected]
Announcement of the results: Within one month from the deadline for applications.
Information clause:
Pursuant to Regulation (EU) 2016/679 of the European Parliament and of the Council of 27 April 2016 on the protection of natural persons with regard to the processing of personal data and on the free movement of such data, and repealing Directive 95/46/EC (hereinafter General Data Protection Regulation - GDPR), the Employer informs that:
- 15 GDPR - access to personal data
- based on Article. 16 GDPR - rectify personal data;
- based on Article. 18 GDPR - request the administrator to restrict the processing of personal data, except to the cases referred to in art. 18 para. 2 GDPR;
- the right to file a complaint to the President of the Office for Personal Data Protection, if the Employee considers that the processing of personal data by the Employer violates the provisions of the GDPR.
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