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to profile tumour and immune cells in melanoma organoid co-cultures and live tissue slices. At Erasmus MC, the candidate will work to combine spatial FUNseq with single-cell genome and transcriptome sequencing
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, molecular and transcriptomic analyses, laser-capture microdissection, and the generation and characterization of lipid nanoparticles. You will work in an interdisciplinary environment combining expertise in
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candidates with experience in one or more of the following areas, cell line editing (CRISPR-KO and knock-in base editing), NK and cytotoxic T cell co-culture assays, single-cell transcriptomics, HLA
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practices in: transcriptomics - designing and executing RNA-seq workflows to characterize cell identity, differentiation, and responses to culture conditions; flow cytometry - implementing and standardizing
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assays, single-cell transcriptomics, and HLA-immunopeptidomics. You will identify how ERAP2 variants and small-molecule modulators alter the peptidome on EBV-infected B cells and how this shifts
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-on-brain.eu/ ). You will employ techniques such as organoid cultures (pluripotent stem cell and tissue-derived), transcriptomic analyses, CRISPR-Cas engineering, and other state-of-the-art tools. Your
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. Beyond the bench, you will take ownership of building part of the methodological backbone of our lab by establishing SOPs and best practices in: transcriptomics - designing and executing RNA-seq workflows
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imaging, spatial transcriptomics/genomics, (multiplex) immunostaining experience with protein-protein interactions and/or working with live snails is a plus affinity for bioinformatics techniques and
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transcriptomics of callus lines and suspension culture; analysing data by bioinformatics and coordinate data exchange; write and publish your research in leading scientific journals, and communicate your findings
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, United States Tina Drinčić Rijskuniverseit Groningen; Universitair Medisch Centrum Groningen The transcriptomic signature of insomnia in major depressive disorder Cousins Center for Psychoneuroimmunology, Semel