Two Postdoc positions in cell signalling, proteomics and glycoproteomics (1.0 FTE)

We are looking for two highly motivated postdoc candidates to work in the area of cell signaling, glycoproteomics, and protein mass spectrometry on the following topics:

Postdoctoral Researcher 1: (PI Maarten Altelaar, [email protected])
Description:
In recent years we have developed new proteomics methods ranging from improved separation and enrichment methodologies to new fragmentation techniques and targeted analysis of post-translation modifications (PTMs). These improvements enable us to decipher the complexity of the human proteome, consisting of dynamic protein-protein interactions, PTMs, protein synthesis and degradation, protein complex assembly and disassembly, etc. To further advance the impact of our proteomics tools in the biological and clinical setting, this project will focus on increasing the sensitivity of existing technologies to enable the analysis of single cell populations. Furthermore, we will aim for smooth integration of the experimental workflows and data analysis with other omics technologies such as genomics and metabolomics.

For this position, we seek a highly motivated Biochemist or Analytical Chemist with proven expertise in mass spectrometry and proteomics. Having your own project, you will team up with dedicated Researchers combining expertise in biochemistry, analytical chemistry, mass spectrometry, and bioinformatics to further develop state-of-the-art proteomics methods that will answer pressing biological questions. As part of the Netherlands X-omics Initiative, you will interact and collaborate with national and international Researchers to enable the efficient integration of proteomics data and workflows with other omics technologies and enable the translation of the integrated Xomics datasets into biologically relevant information.

We are looking for a driven candidate who fits well with the following qualifications:

• experience in operating high-end LC-MS systems;
• experience with sensitive (phopsho)proteomics sample preparation protocols;
• experience in handling large data sets and statistical analysis;
• experience with mammalian cell culture (considered an advantage);
• programming skills or R-experience (considered an advantage);
• excellent spoken and written English language skills.

Postdoctoral researcher 2: (PI Karli Reiding, [email protected])
Postdoctoral research project in Biomolecular Mass Spectrometry and Proteomics: Proteomics applications to study the remodeling of neutrophils in cancer and in health.
Description:
Neutrophils are highly abundant and important cells in the immune system. Yet, while most neutrophils are anti-pathogenic, a subset thereof is able to suppress T-cell activation. These so-called myeloid-derived suppressor cells (MDSCs) often reside in the tumor microenvironment and prevent an effective immune response. Interestingly, it has been demonstrated that IgA is not only capable of directing neutrophils to attack tumor cells, but also that antibodies can significantly diminish the suppressive effects of MDSCs. However, the molecular basis of these effects remains unclear and needs to be investigated further.

The aim of this project is to develop and apply mass spectrometry (MS) technologies to characterize conventional and immunosuppressive neutrophils, to determine how these respond to immunoglobulin stimulation, and to find out how these differ within health and disease. Required techniques will include proteomics to determine protein abundances, as well as dedicated MS strategies to investigate post-translational modifications like glycosylation and phosphorylation.

For this position, we seek a highly motivated Biochemist or Analytical Chemist with experience in mass spectrometry, proteomics, and sample preparation methods. Having your own project, you will team up with dedicated Researchers combining expertise in biochemistry, analytical chemistry, mass spectrometry, and bioinformatics to further develop state-of-the-art proteomics methods that will answer pressing biological questions. Specifically, we request the following qualifications:

• experience with operating high-end LC-MS systems;
• experience with proteomics sample preparation methods;
• experience with glycoproteomics and/or phosphoproteomics (considered an advantage);
• experience with handling large data sets and statistical analysis (considered an advantage);
• programming skills or R-experience (considered an advantage);
• excellent spoken and written English language skills.