This project is part of a consortium that aims to unravel hereditary anemias using induced pluripotent stem cells (iPSCs) for the purpose of gaining more fundamental knowledge and novel therapies. This consortium focuses on hematopoietic stem cells (HSCs). Mature blood cells are continuously replenished by HSCs to make sure that essential mechanisms remain functional, such as oxygen tranport and defence again pathogens. The production of new blood cells is impaired in a plethora of diseases. Blood transfusion is often the only therapeutic option and stem cell transplantation the only curative treatment. To obtain viable therapies, it is essential to build up know-how on large scale cultivation of iPSC-derived autologous HSC transplantation production. The TU Delft contributes to this consortium with two PhD positions of which one is described in this vacancy. The PhD position in this vacancy is experimental and will establish HSC production at lab-scale. The second PhD position is computational and focussed on employing computational fluid dynamic simulations to design bioreactor cultivations.
The PhD project focuses on the development of bioreactor cultivation systems and protocols to standardize the expansion and differentiation of iPSCs to HSCs. This work will contribute to establish a viable therapeutic production process. Insights obtained in this project will be employed to better understand the effects of HSC cultivation and identify novel approaches to control the cultivation of HSCs. You will collaborate closely with Sanquin Blood Supply Foundation and Applikon Biotechnology BV.
Key focus points
- Build a lab-scale bioreactor setup to cultivate HSCs from iPSCs (including cultivation training at Sanquin).
- Optimize process parameters on lab-scale to ensure cultivation reproducibility and robustness.
- Unravel the effects of critical process parameters during different cultivation stages.
- Identify approaches to facilitate model-based cultivation control.
You will have the opportunity to further develop your scientific career by 1) gaining hands-on experience with cultivation scale-up for cell therapy modalities, 2) expanding your skill set with process optimization and control, and 3) the ability to apply creativity and conceptual thinking to answer pressing research questions for this novel bioprocess. In addition, you will participate in educational activities as a (MSc or BSc) course assistant and supervisor of BSc/MSc graduation projects.
The position will be in the research group Applied Analytical Technology and Data-Driven Bioprocess Design which is part of the Bioprocess Engineering section at the Department of Biotechnology. This group focusses on unravelling the connection between raw materials, process parameters, and product quality, and using that information to optimize or design for a wide variety of bioprocesses.
Ph D Position: Computational Analysis Of Stem Cell Production In Bioreactors, Delft University of Technology (TU Delft), Netherlands, 30 days ago
Blood is essential for oxygen transport, wound healing, and defence against pathogens. Short-lived mature blood cells are constantly replenished by hematopoietic stem cells (HSCs) throughout life....
Ph D Position: Computational Analysis Of Stem Cell Production In Bioreactors, Delft University of Technology, Netherlands, about 3 hours ago
Stem cell therapies hold great promise when it comes to curing blood diseases - will your process insights ensure we can culture enough cells to make these therapies reality? Blood is essential fo...
Ph D Student B Cell Differentiation, Sanquin Blood Supply Foundation (Sanquin), Netherlands, about 7 hours ago
The program investigates regulation of antibody responses to be able to prevent occurrence of unwanted antibody responses against red blood cells (RBCs), platelets or other blood products. The pos...
Ph D Position (M/W/D), E13/65 % , Technical University of Munich, Germany, about 7 hours ago
01.06.2022, Wissenschaftliches Personal We are looking for a scientist (PhD student, m/w/d) who shares our enthusiasm about human T cell engineering and to work on our new project entitled “CRISPR...
Doctoral (Ph D) Student Position In Blood Engineering, Karolinska Institute, Sweden, 9 days ago
To be a doctoral student means to devote oneself to a research project under supervision of experienced researchers and following an individual study plan. For a doctoral degree, the equivalent of...
Ph D In Biomedical Engineering / Mechanobiology (# Of Pos: 2), Trinity College Dublin, Ireland, 15 days ago
Two funded PhD studentships have become available in the area of ocular mechanobiology. The aim of these projects will be to explore the effect of fluid flow and mechanical environment on the beha...