PhD position in biophysics: Cell division at the nanoscale (1.0 FTE)

Updated: 2 months ago
Job Type: Temporary
Deadline: 29 Aug 2022

We are looking for a PhD candidate with a biophysics background. The aim of this PhD project is to develop (live-cell) STED super resolution microscopy methods to visualize how the DNA copies are separated during cell division. Affinity for wet-lab work, and experience in general programming (Python) and optics are desirable.

You will have the following main responsibilities and tasks:

• develop protocols for optimal labelling of cell-division proteins for STED and expansion microscopy
• develop image analysis algorithms using Python
• investigate minimal impact imaging protocols.

We are studying how protein structures change during cell division with STED microscopy. Cell division is a very dynamic process, and errors in this phase of the cell cycle have far-reaching consequences. Visualizing the protein dynamics at relevant scales is not trivial, as the dynamical interplay is at scales much smaller than the diffraction limit of light, and thus beyond the reach of traditional microscopes. STED microscopy however does allow imaging with a resolution of 30-40 nanometres even in living cells. In fixed cells, the combination with STED and expansion microscopy even reaches better resolutions. To successfully study this dynamic process is challenging.

With your biophysics background, and affinity for programming and wet-lab work, you are the ideal fit to lift our research to another level! The work will be diverse, including (live-cell) sample preparation, protocol optimization, measurements and software development for data analysis.

You will work in an interdisciplinary and international research environment with local colleagues and international collaborators from physics, biomolecular sciences, and informatics.

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