PhD in de novo design of ice-binding proteins

Eindhoven University of Technology (TU/e), Netherlands

Updated: over 1 year ago
Job Type: Temporary
Deadline: 02 Dec 2022

  • Are you interested in (de novo) protein design?
  • Are you fascinated by ice crystal growth?
  • Would you like to know more about antifreeze proteins and cryobiology?
  • Are you passionate about research?
  • Do you want to work in a multidisciplinary team?

    • JobDescription

    Ice-binding proteins (IBPs) protect against freeze damage in polar regions by keeping the nucleation and growth of ice crystals in check. How this works exactly is not yet known. You will engineer IBPs de novo, express and study these to elucidate the relation between IBP structure and function and explore the application potential of IBPs as cryoprotectants. In this project you will combine computational tools for protein engineering (de novo protein design, folding) with biochemical and structural biology techniques (protein expression, structure elucidation, activity assays) to modulate independently the various hallmarks of IBP activity. These include the ability of your novel ice-binders to depress freezing, inhibit recrystallization, control nucleation, and shape ice crystals. In collaboration with group members and external collaborators, you will investigate ice-binding of your designs at the single molecule level as well as their application potential in biomedicine focusing on e.g. cryopreservation of heart cells and tissues.


    Similar Positions