PhD candidate studying early lineage decisions in embryonic development using 3D stem cell models

Are you fascinated by mammalian embryonic development and stem cells and do you enjoy doing experiments? Are you excited by the idea of combining imaging and molecular analyses to study developmental signaling pathways in a 3D in vitro stem cell model? Then this may be the job for you! 

We are looking for an enthusiastic and intrinsically motivated PhD candidate who is eager to study how stem cells respond to developmental signals using gastruloids as a model system. The position is supported by a recently awarded starters grant and you will be embedded in the Developmental, Stem Cell and Cancer Biology group, which is part of the Swammerdam Institute for Life Sciences in the Faculty of Science at the University of Amsterdam. Research in our group aims to understand how complex tissues are built and maintained. We study this at multiple different levels – “from man or mouse to molecule” – and with a specific focus on embryonic signaling. See our website for more details. 

What are you going to do?

The early mammalian embryo is composed of pluripotent stem cells that can generate all cell types of the future body. At the start of gastrulation, instructive signals belonging to the WNT, NODAL and FGF signaling pathways, determine which developmental trajectory the cells will take. How these signaling molecules lead to different cell types over time is not well understood.

Your focus will be on studying how the interplay between these different signaling pathways controls the first lineage decisions in development. For this, you will use live cell imaging and cell tracking in a 3D model of early embryogenesis (i.e.  “gastruloids”). You will also focus on how stem cells receiving these signals interpret signaling through specific gene regulatory networks.

During your PhD research, you will learn and apply a variety of techniques, including (but not limited to) DNA cloning, 2D stem cell culture and targeting using CRISPR/Cas9, 3D gastruloid culture, live-cell confocal microscopy, and manipulation of gastruloids using a combination of classical developmental biology and state-of-the-art genetic perturbation approaches. 

Tasks and responsibilities:
•    complete and defend a PhD thesis within the official appointment duration of four years;
•    use state of the art molecular cell biology approaches to visualize, manipulate and track individual cells in 3D;
•    perform your experiments in a systematic and well controlled manner;
•    keep accurate records by thoroughly documenting and organizing your work;
•    discuss your work in lab meetings, incorporate feedback and give input to others;
•    assist in teaching and supervise BSc/MSc students during their research internship;
•    participate in the Faculty of Science PhD training programme.

You will get the opportunity to:
•    learn and apply a wide variety of molecular, cell biology and imaging techniques;
•    learn, develop and apply quantitative image analysis;
•    present your work at (inter)national meetings;
•    continuously hone your academic, professional and personal skills.

What do you have to offer?

You are an enthusiastic and meticulous researcher who is excited by developmental and/or stem cell biology. You like a challenge, take initiative, and are motivated to work in an interdisciplinary environment. You are well organized, enabling you to meet deadlines. Finally, you are a team player and a nice colleague with good communication and problem-solving skills.

Your experience and profile 
You have/are:
•    a high intrinsic motivation for fundamental research;
•    eager to learn new techniques;
•    a recent MSc degree in the biomedical sciences (or a related area in molecular and/or cell biology);
•    proficient in mammalian tissue culture;
•    affinity with confocal microscopy and image analysis;
•    fluent in English, both written and spoken.

If you are genuinely excited about your project and willing and able to learn, we can teach you everything you need to know. However, it will work to your advantage if you already have experience with one or more of the following: (embryonic) stem cell culture, DNA cloning, confocal microscopy, image analysis (e.g. FIJI, CellPose), bioinformatics analyses and/or computational biology (e.g. R, Python, Matlab). 

Our offer

A temporary contract for 38 hours per week for the duration of 4 years (the initial contract will be for a period of 18 months and after satisfactory evaluation it will be extended for a total duration of 4 years). The preferred starting date is 1 May 2023 but we are flexible. We will draft an educational plan that includes attendance of courses and (international) meetings. We also expect you to assist in teaching undergraduates and master students for a maximum of 10% of your time.

Your appointment will be at the University of Amsterdam, where you will be co-supervised by experts in developmental signaling and early embryonic development (dr. Thijs van Boxtel and dr. Renée van Amerongen) and quantitative image analysis (dr. Marten Postma).

Your salary will range between €2,770 in the first year to €3,539 gross per month in the last year of employment, on the basis of a full working week of 38 hours. This sum does not include the 8% holiday allowance and the 8.3% year-end allowance. A favorable tax agreement, the 30% ruling, may apply to non-Dutch applicants. The Collective Labour Agreement for Dutch Universities (CAO NU) is applicable. 

Besides the salary and a vibrant and challenging environment at Science Park, we offer you multiple fringe benefits:
•    232 holiday hours per year (based on fulltime);
•    multiple courses to follow from our Teaching and Learning Centre;
•    a complete educational program for PhD students;
•    a pension at ABP for which UvA pays two third part of the contribution;
•    the possibility to follow courses to learn Dutch;
•    help with housing for a studio or small apartment when you’re moving from abroad. 

Are you curious to read more about our extensive package of secondary employment benefits, take a look here .

About us

The University of Amsterdam is the Netherlands' largest university, offering the widest range of academic programmes. At the UvA, 30,000 students, 6,000 staff members and 3,000 PhD candidates study and work in a diverse range of fields, connected by a culture of curiosity.

The Faculty of Science has a student body of around 8,000, as well as 1,800 members of staff working in education, research or support services. Researchers and students at the Faculty of Science are fascinated by every aspect of how the world works, be it elementary particles, the birth of the universe or the functioning of the brain. 

The Swammerdam Institute for Life Sciences is one of eight institutes in the Faculty of Science (FNWI). SILS is a multidisciplinary research institute with a systems biology approach to the life sciences. The institute brings together approximately 240 researchers in 18 research groups. 

Want to know more about our organisation? Read more about working at the University of Amsterdam.

Any questions?

Do you have any questions or do you require additional information? Please contact:

                T: +31 20-525 6225

Job application

If you feel the profile fits you, and you are interested in the job, we look forward to receiving your application. You can apply online via the button below. We accept applications until and including 31 January 2024, but we may contact suitable candidates prior to this date. 

Applications should include the following information (all files should be submitted in one single pdf file):

•    a letter of motivation;
•    a detailed CV including the months (not just years) when referring to your education and work experience;
•    the names and contact information of two references (no letters of recommendation are required at this stage)

Only complete applications received within the response period via the link below will be considered.

A knowledge security check can be part of the selection procedure.
(for details: National knowledge security guidelines ).

The UvA is an equal-opportunity employer. We prioritise diversity and are committed to creating an inclusive environment for everyone. We value a spirit of enquiry and perseverance, provide the space to keep asking questions, and promote a culture of curiosity and creativity.

If you encounter Error GBB451, reach out to our HR Department  directly. They will gladly help you continue your application. 

No agencies please.