PhD Candidate | Redirecting cardiac wound healing towards regeneration | 1.0 FTE

PhD Candidate | Redirecting cardiac wound healing towards regeneration | 1.0 FTE
PhD Candidate | Redirecting cardiac wound healing towards regeneration | 1.0 FTE

Published	Deadline	Location
17 May	4 Jun	Maastricht

Would it not be great to repair a damaged heart, rather than treating the consequences of this damage with lifelong medication? Myocardial infarction is still one of the most frequent cardiovascular events and a common cause of heart failure. Presently, the wound healing process evoked by cardiac injury leads to formation of a scar, which does not contribute to the cardiac output. The central aim of this project is to design strategies to redirect this wound healing towards cardiac regeneration, thereby restoring the function of the heart and preventing the development of heart failure.
Job description

Adult cardiomyocytes are terminally differentiated cells, with a very low rate of proliferation in adult mammals. During normal wound healing after myocardial infarction, this low proliferation rate prevents regeneration of the lost cardiac tissue, shifting the balance towards a massive fibrotic response. Therefore, investigations aiming to redirect the wound healing process away from scar formation, into the direction of cardiac regeneration have high scientific and societal potential.

Previous research has shown that several signaling pathways, including WNT and TGF-beta signaling, are associated with reduced scar formation and induction of cardiac regeneration in zebrafish and new-born mice. However, inhibiting the fibrotic response is not without risk, as it may compromise the mechanical integrity of the infarct area and contribute to dilatation of the ventricles.

This PhD position is part of a recently funded ZonMW Open competition project entitled “The NEOHEART study: redirecting the wound healing after myocardial infarction towards cardiac regeneration by combining mechanical and pharmacological support”. This project is a collaboration between researchers from Leiden UMC, TU Eindhoven and Maastricht University, combining expertise in cell biology, biomaterials and pharmacology. The PhD student will benefit from this synergistic to be successful in completing this project.

The novelty of this project is that we will combine pharmacological stimulation of cardiac regeneration with mechanical support at the site of the infarct, to avoid dilatation of the ventricles or even rupture of the infarct area. To this end, we will develop a supramolecular fibrous mesh material that combines mechanical support of the infarct area with drug-eluting properties. This approach allows achieving pharmacologically relevant concentrations of drugs in the infarct area, without excessive systemic exposure that could cause side effects. The pharmacological and mechanical interventions will be tested in 3-dimensional co-cultures of cardiomyocytes and fibroblasts and on heart slices, before proof of concept is obtained in relevant animal models of MI. The PhD student located at UM will specifically focus on optimizing the 3-dimensional co-cultures and the testing of substances that promote the cycle of dedifferentiation, proliferation and redifferentiation required for regeneration.

Further details about the project can be found here: https://www.zonmw.nl/sites/zonmw/files/typo3-migrated-files/Gehonoreerde_projecten_Open_Competitie_ronde_2021_NL_en_EN.pdf 

Specifications

• max. 38 hours per week
• €2541—€3247 per month
• Maastricht View on Google Maps

Maastricht University (UM)

Requirements

Are you excited to work in an international project team for an origination that is of societal significance? Are you self-motivated, curious and interested in research?
Do you have the skills required to become an excellent researcher in your field? Then a PhD position at CARIM might be the challenge you are looking for.

Candidates will be selected on the basis of the requirements outlined below. To be considered, candidates should make sure that their application demonstrates how they meet these requirements:

We are looking for a PhD-candidate with the following qualifications: 

• A MSc. degree in Biomedical Sciences, Molecular Life Sciences or a related field is required;
• You have a real researcher’s mentality: open-minded, curious, enthusiastic, inquisitive and accurate;
• Excellent scientific English writing and presentation skills are needed to work effectively within our challenging multidisciplinary research team;
• You have critical thinking and problem solving skills, you are flexible, can set priorities and show ethical conduct;
• A theoretical background in- and affinity with stem cell biology and pharmacology is an advantage;
• Hands-on experience with common laboratory techniques such as cell culture, RT-PCR, Western blotting, and immunocytochemistry would work in your favor.

Conditions of employment

We offer a rewarding career at a young university in the heart of Europe, with a distinctly global perspective and a strong focus on innovative research and education.
We provide a stimulating, collaborative and interdisciplinary work environment with excellent opportunities for professional and personal development.
Maastricht University has an excellent international reputation, and Maastricht itself is a beautiful, open-minded city located in the center of Western Europe.

• We offer a 4-year full-time appointment as a Ph.D. candidate. The first year will be a probation period; after a positive assessment, the position will be extended for another 3 years.  Start date of the position is August / September 2023.
• Remuneration will be according to standard salary levels for PhD students starting with a salary of € 2,541 with a yearly growth to € 3,247 gross a month (based on a full-time appointment).
• Each year the standard salary is supplemented with a holiday allowance of 8% and an end-of-year bonus of 8.3% of the yearly gross income.
• We offer an attractive package of fringe benefits such as a reduction on collective health insurance, substantial leave arrangements, an optional model for designing a personalized benefits package, sports facilities that staff may use at a modest charge, and application for attractive fiscal arrangements for employees from abroad.
• You have to be willing to move to (the vicinity of) Maastricht. If you do not already live in Maastricht (or its direct surroundings), you will be eligible for an allowance for moving costs and relocation reimbursement.

The terms of employment of Maastricht University are set out in the Collective Labour Agreement of Dutch Universities (CAO). Furthermore, local UM provisions also apply. For more information look at the website www.maastrichtuniversity.nl  > About UM > Working at UM .

Employer
Maastricht University

Maastricht University is renowned for its unique, innovative, problem-based learning system, which is characterized by a small-scale and student-oriented approach. Research at UM is characterized by a multidisciplinary and thematic approach, and is concentrated in research institutes and schools. Maastricht University has around 22,000 students and about 5,000 employees. Reflecting the university's strong international profile, a fair amount of both students and staff are from abroad. The university hosts 6 faculties: Faculty of Health, Medicine and Life Sciences, Faculty of Law, School of Business and Economics, Faculty of Science and Engineering, Faculty of Arts and Social Sciences, Faculty of Psychology and Neuroscience. 
For more information, visit www.maastrichtuniversity.nl .

Department
CARIM – School for Cardiovascular Diseases

CARIM is one of the six schools of the Faculty of Health, Medicine and Life Sciences (FHML) of Maastricht University and is embedded within the Maastricht University Medical Center+ (Maastricht UMC+). CARIM collaborates closely with the Heart+Vascular Center of Maastricht UMC+. CARIM is one of the largest cardiovascular research institutes in Europe, producing more than 500 scientific articles and approximately 40 PhD dissertations per year.

Further information is available at the CARIM website: https://www.carimmaastricht.nl/

Department of Pharmacology & Toxicology
This project is situated within the Department of Pharmacology & Toxicology where a multidisciplinary team of biologists, chemists and toxicologists is working together to address mechanisms by which environmental chemicals and oxidative stress alter cellular processes to cause diseases and study the way in which intervention is possible. To reach these goals, the department's laboratory is well equipped with facilities for chemical analysis including mass spectrometry (MS) and electron spin resonance (ESR), the analysis of DNA damage and multiple state-of-the-art molecular biological techniques. The research is carried out on a (bio) chemical, cellular, tissue or whole organism level. The department consists of 16 staff members (4 professors, 3 senior lecturers and 9 lecturers/postdocs), 6 support technicians and 15 PhD students.

https://phartox.nl/general-information

Additional information

For more information regarding this vacancy, you can contact:
Dr. W. Matthijs Blankesteijn, e-mail [email protected]

Interview dates: The interviews will take place in week 23 and 24, please indicate any days on which you are not available.

 The preferred starting date of this PhD-project is August/September 2023.

 To apply for this vacancy, press the button ‘apply now’ to see the application procedure.

Working at Maastricht University

Maastricht University distinguishes itself with its innovative education model, international character and multidisciplinary approach to research and education.

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Apply via postal mail
Apply via postal mail

[email protected]

Don't forget to mention AcademicTransfer and the job number: AT2023.233 in your letter.

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Application procedure

You can respond to this vacancy by sending your motivation letter and CV (as pdf file), stating the vacancy number AT2023.233, by email to:
[email protected]

Maastricht University is committed to promoting and nurturing a diverse and inclusive community. We believe that diversity in our staff and student population contributes to the quality of research and education at UM, and strive to enable this through inclusive policies and innovative projects led by teams of staff and students. We encourage you to apply for this position.

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