Senior Scientist / Postdoc (PhD) (m/f/d) CRISPR-mediated gene therapy

13.05.2022, Wissenschaftliches Personal

The Research Unit Translational Immunology (TUM) in collaboration with the Institute of Lung Health and Immunity (LHI; Helmholtz Munich) is looking for a senior scientist / postdoc (PhD) (m/f/d) to study pulmonary delivery of gene editing tools and successful genetic and functional correction of STAT3 mutations in lung cancer cells and iPSC-derived alveolar epithelial cells.

The Research Unit Translational Immunology (TUM) and the Institute of Lung Health and Immunity (LHI; Helmholtz Munich) joined forces to better understand and cure lung diseases associated with a dysregulated STAT3 signaling using CRISPR-mediated gene editing. The proposed project focuses on STAT3 gene correction in lung cancer and STAT3-hyper-IgE syndrome (STAT3-HIES), which is a monogenic inborn error of immunity with chronic destructive lung disease. To correct the underlying disease-causing STAT3 mutations we established CRISPR/Cas9-mediated base editing (Eberherr et al. CRISPRJ).

Your responsibilities:

• Establish air-liquid interface (ALI) culture of iPSC-derived alveolar epithelial cells
• Perform gene editing of human lung cancer cells and iPSC-derived alveolar epithelial cells using polymers developed at the lab of Prof. Merkel (Drug Delivery, LMU)
• Assess efficiency and safety of the delivery method and the performed CRISPR-mediated gene editing

Your profile:

• PhD in one of the life sciences or equivalent work experience in the wet lab
• A strong background in polymer-based cellular delivery of nucleic acids or proteins and cell culture technique is favorable
• Ideally you have experience in gene editing (including gRNA design and validation), ALI and/or iPSC culture
• Experience in downstream analysis including immunofluorescence staining, flow cytometry, western blot analysis, quantitative real-time PCR, Sanger sequencing, high-throughput sequencing, in silico analysis of editing efficiency, cell viability analysis is a plus
• The highly motivated candidate must be able to work independently however we greatly seek team player mentality

We offer you:

• The Technical University of Munich (TUM) is one of the leading research universities in Europe.
• We are offering excellent working conditions and an international research environment.
• The project will be conducted at the Munich-Neuherberg campus of Helmholtz Munich.
• The project is initially limited to two years. An extension of the project is planned.  

If the candidates’ suitability for the position in question is equal, severely disabled applicants shall be given preference. Interview-related costs can, unfortunately, not be reimbursed.

We look forward to your application!

Please submit your complete application documents (letter of motivation, CV, (degree) certificates, at least two references) by e-mail by 05.06.2022 at the latest. 

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Kontakt: Prof. Dr. med. Ellen Renner; E-mail: [email protected]

https://www.tiem.med.tum.de/en