The application of MSC as cell therapeutics is a growing sector in health care. MSC therapeutics contain ~ 10^8 cells per dose, an amount that cannot solely be isolated from donor tissue. Thus, an in-vitro MSC expansion process is necessary and crucial, as it has a high impact on the quality of the final MSC product. The main goal of the project is the development of a novel dissolvable microcarrier that enables the adhesion and growth of mesenchymal stem cells (MSC) in a bioreactor process, and an efficient cell harvest while preserving the therapeutic potential of MSC. The porous, starch-based microcarriers will be optimized to ensure a mechanical support and a high surface-to-volume ratio when applied in a bioreactor. It will be dissolvable to enable a harvesting procedure that avoids a proteolytic detachment of the cells. A novel coating of the microcarriers will combine synthetic, bioactive peptides and glycosaminoglycans. The coating will be MSC-specific and will enable the adhesion and survival of the anchorage-dependent MSC in a serum-free cell culture medium, and support the preservation of their functionality. The microcarriers will be applied in a stirred-tank bioreactor. Process parameters promoting an optimal yield and MSC functionality will be identified and optimized in a design-of-experiments (DOE) approach. Physicochemical, cell-associated, and equipment-associated process parameters were investigated to determine critical ones. A limited fed batch model will be developed and then coupled with an online monitoring of the cell biomass via impedance spectroscopy. This process control strategy will be tested in various scenarios and for different MSC cell types. The novel microcarrier prototypes are tested in a real MSC expansion process and investigated regarding their sedimentation behaviour and their influence on mixing time and mass transfer processes, and analysed regarding the adhesion, growth, and the harvest of the MSC in the stirred tank reactor.
Postdoc Position In Biofabrication Enlight, University Medical Center Utrecht (UMC Utrecht), Netherlands, 13 days ago
- You hold a PhD degree in biofabrication, synthetic biology, biomedical engineering, regenerative medicine or in a related area - Proven expertise in biomaterial-based (stem) cell 3D culture and ...
Postdoc Position Mechanobiology Of Bioengineered Bone Organoids , ETH Zurich, Switzerland, about 15 hours ago
In der aktuellen Covid-19 Situation laufen die Rekrutierungen weiter. Es kann dabei allerdings zu Verzögerungen kommen. Vielen Dank für Ihr Verständnis. 100%, Zurich, fixed-term The Laboratory for...
Postdoctoral Studies In Dna Damage And Ubiquitin Signaling (Scholarship), Karolinska Institutet, Sweden, about 5 hours ago
Do you want to contribute to top quality medical research? The Department of Cell and Molecular Biology (CMB, https://ki.se/en/cmb/department-of-cell-and-molecular-biology) is one of five departme...
Ph D Candidate For Epigenetic Regulation Of Early Human Embryonic Development, Radboud University, Netherlands, 1 day ago
A PhD Candidate in the context of the Marie Curie ITN network EpiSyStem is available to study epigenetic regulation of gene expression in differentiated embryonic stem cells. Various omics approac...
Postdoc Position: Molecular Mechanisms Of Angiogenesis In Ocular Diseases, Academic Medical Center (AMC), Netherlands, about 6 hours ago
What you are going to do The Ocular Angiogenesis Group is looking for a motivated candidate to join our team and study the molecular mechanisms of angiogenesis in ocular diseases. Angiogenesis or ...
Postdoctoral Position In Immunology/Oncology, University of Alberta, Canada, about 3 hours ago
The Gamper Lab at the Cross Cancer Institute is seeking an outstanding cell biologist, immunologist, or biochemist for a postdoctoral position with an immediate start (with some flexibility). The ...