PhD Position - Cryo-EM of membrane remodeling proteins

Updated: 3 months ago
Location: Germany,
Deadline: 10 Oct 2022

Work group:

ER-C-3 - Strukturbiologie

Area of research:

PHD Thesis

Job description:

Your Job:

For the Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons under the direction of Prof. Sachse, you will be working on cryo-EM studies of membrane remodelling proteins in the field of Structural Biology at our institute. The Sachse lab recently determined a series of cryo-EM structures of bacterial as well as eukaryotic members of the endosomal sorting complexes required for transport (ESCRT) protein family. In order to gain a deep structural understanding, we biochemically purify and reconstitute ESCRT protein complexes in lipid environments and visualize them by cryo-EM methods. You will be working in a network of membrane biological research and latest cryo-EM infrastructure.

  • Investigate protein lipid interactions using biochemical and biophysical methods
  • Employ advanced imaging methods, including single-particle cryo-EM, electron tomography, correlative light and electron microscopy/tomography (CLEM) as well as associated image processing.
  • Cryo-EM structure determination in vitro and in situ

Your Profile:

  • Master degree in biochemistry, molecular or cell biology or related field.
  • Strong experimental skills in molecular cell biology experiment design including associated data analysis
  • Prior knowledge of structural biology and/or light microscopy techniques is of great advantage
  • Strong communication skills and ability to work in an international and interdisciplinary team
  • Fluent command of written and spoken English, German language skills are welcome but not essential

Our Offer:

We work on the very latest issues that impact our society and are offering you the chance to actively help in shaping the change! We offer:

  • The chance to work at one of the largest research centers in Germany, with excellent scientific equipment and leading European computational resources, located on a green campus, and near the cultural centers Köln, Düsseldorf, and Aachen. The Jülich campus also hosts a vibrant biophysics, bioinformatics and structural biology community.
  • Direct access to high-level cryo-EM infrastructure at the Ernst-Ruska Centre. The facility has been extended with state-of-the-art cryo-microscopes and FIB-SEMs of ThermoFisher Titan Krios, Talos Arctica and Aquilos 2.
  • Working in a dynamic team of researchers with backgrounds in different disciplines across biology, chemistry, physics and informatics to advance cryo-EM methods
  • Further development of your personal strengths, e.g. through an extensive range of training courses; a structured program of continuing education and networking opportunities specifically for doctoral researchers via JuDocS, the Jülich Center for Doctoral Researchers and Supervisors:
  • Targeted services for international employees, e.g. through our International Advisory Service

The employment of doctoral researchers at Jülich is governed by a doctoral contract, which usually has a term of three years. Pay is in line with 65 % of pay group 13 of the Collective Agreement for the Public Service (TVöD-Bund) and additionally 60 % of a monthly salary as special payment („Christmas bonus“). Further information on doctoral degrees at Forschungszentrum Jülich including our other locations is available at:

We welcome applications from people with diverse backgrounds, e.g. in terms of age, gender, disability, sexual orientation / identity, and social, ethnic and religious origin. A diverse and inclusive working environment with equal opportunities in which everyone can realize their potential is important to us.

This research center is part of the Helmholtz Association of German Research Centers. With more than 42,000 employees and an annual budget of over € 5 billion, the Helmholtz Association is Germany's largest scientific organisation.

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