Master’s and Ph.D. position on: Biological engineering of gene reporters for Electron Microscopy of brain circuits

09.11.2020, Wissenschaftliches Personal

Synthetic Biology project on generating GFP for Electron Microscopy via genetic- and protein-engineering of EM-visible nanostructures for neuroscience applications.

Is your mind focused on synthetic biology, and do you want to contribute to unraveling how memories are encoded in brain circuits using molecular imaging at the best imaging resolution? Then you are likely interested in a recently ERC-funded project of our laboratory (westmeyerlab.org) at the Munich School of Bioengineering (https://www.bioengineering.tum.de/) of TUM.

Our team focuses on generating "GFP for Electron Microscopy," i.e., gene reporters for Electron Microscopy (EM) to help unravel how brain activity and neuronal connections are interlocked. Specifically, we will use encapsulins [references 1,2] as the lead structures for genetically encoded self-assembling nanostructures that can be visualized by high-throughput EM of serial brain sections. In this way, we can directly overlay molecular information about, e.g., synaptic plasticity onto the brain wiring diagram ("connectome") at EM resolution in loose analogy to how google maps displays traffic information on top of the road map. This novel reporter technology will accelerate the systematic analysis of function-structure relationships in brains with implications for our understanding of neuropsychiatric diseases and inspiration for neuromorphic computing.

Your Profile an excellent and recent Bachelor's or Master’s degree in synthetic biology, biological engineering, biochemistry, biophysics, neurobiology, biomedical engineering or related academic programs, excellent knowledge in molecular biology, advanced cloning, and mammalian cell culture, a keen interest in molecular imaging including Electron Microscopy, as well as neurobiology, a strong interest in de novo protein design, the ability to be self-motivated and work with an interdisciplinary team of bioengineers, biochemists, neuroscientists, and data scientists, excellent English language and organizational skills. an openness and mobility to collaborate with our partners at Max Planck Institutes, MIT, Caltech, and the University of Washington.

TUM The Technical University of Munich (TUM) combines top-class facilities for cutting-edge research with unique learning opportunities for students. It is committed to finding solutions to the major challenges facing society as we move forward: Health & Nutrition • Energy & Natural Resources • Environment & Climate • Information & Communications • Mobility & Infrastructure. The university thinks and acts with an entrepreneurial spirit. Its aim: to create lasting value for society. All this combines to make it one of Europe's leading universities.

Applications from disabled persons with essentially the same qualifications will be given preference. TUM strives to raise the proportion of women in its workforce and explicitly encourages applications from qualified women.

Please send your letter of motivation and your complete CV to ga49qal@mytum.de.

Please also note: https://portal.mytum.de/kompass/datenschutz/Bewerbung/.

References: [1] Sigmund, F., Pettinger, S., Kube, M., Schneider, F., Schifferer, M., Schneider, S., Efremova, M.V., Pujol-Martí, J., Aichler, M., Walch, A., Misgeld, T., Dietz, H., Westmeyer, G.G., 2019. Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene Reporters. ACS Nano 13, 8114–8123. doi:10.1021/acsnano.9b03140

[2] Sigmund, F., Massner, C., Erdmann, P., Stelzl, A., Rolbieski, H., Desai, M., Bricault, S., Wörner, T.P., Snijder, J., Geerlof, A., Fuchs, H., Hrabe de Angelis, M., Heck, A.J.R., Jasanoff, A., Ntziachristos, V., Plitzko, J., Westmeyer, G.G., 2018. Bacterial encapsulins as orthogonal compartments for mammalian cell engineering. Nat Commun 9, 1990. doi:10.1038/s41467-018-04227-3

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Kontakt: ga49qal@mytum.de

http://www.westmeyerlab.org