PhD scholarship in Fast Volumetric Two-Photon in vivo Imaging

Updated: 2 months ago

The Department of Health Technology at the Technical University of Denmark (DTU) is seeking a candidate for a PhD position on “Fast Volumetric Two-Photon in vivo Imaging”.

The PhD student will join the Biophotonic Imaging group at the Department of Health Technology (DTU Health Tech) consisting of ca 10 employees, including several PhD students and postdocs. Within the group, we are working on the advancement of optical imaging technologies for clinical and biological applications. Currently, we have research projects on multimodal imaging combining optical coherence tomography, two-photon excitation fluorescence microscopy, light sheet microscopy, optical probe head development, and investigation of biomarkers relevant for cancer diagnosis using biophotonic imaging.

Background
Visualizing dynamic biological processes in their native environment leads to better understanding of cell and tissue function. Furthermore, live in vivo monitoring of disease formation at the earliest onset paves the way for new or improved treatment and drug development. The aim to provide functional imaging of such processes in their native biological environment is the major driver for the development of cutting-edge, fast optical imaging tools in photonics labs all around the world.

A wide range of researchers in biology and the health sciences could be empowered to use these new tools if these systems were robust to allow transport and handling while maintaining alignment without the need for intervention by technical staff and at the same time were easy to operate. This can be provided by implementing optical imaging modalities in all-fibre based systems. They must provide accurate information sought by the biologists, preferably in three dimensions from pristine samples, by probing the appropriate biomarkers, such as morphology, microstructure or chemical composition. This can be fulfilled by implementing multimodal imaging, combining the best suited optical modalities in a single platform, and by automated processing of the gathered data. And they must allow fast imaging to allow monitoring of dynamic processes. This can be implemented by clever imaging geometries and compressed sensing.

Fulfilling these goals will allow an unprecedented snapshot into various conditions and indeed is a prerequisite if we wish to perform early intervention, diagnosis and ultimately improve treatment in numerous conditions including cancer, immunotherapies, and neurodegenerative diseases amongst others.

Responsibilities and topics
This PhD project aims at delivering a step change towards the above-mentioned goals in two-photon imaging. Your objective will be to implement fast two-photon excited fluorescence microscopy using beam shaping with an SLM in free space and through a multicore fibre, and in a miniaturised probe head using micro-optical elements for in vivo testing in small animals. You will explore the possibilities of compressive sensing for point-scanned and light sheet two-photon imaging, implement computer models to quantitatively analyse the data and show the performance on in vitro, ex vivo and in vivo samples.

Each of the above topics could result in a publication:

  • fast two-photon imaging providing relevant biomarkers on spheroids, porcine colon samples, and glioblastoma samples;
  • compressive sensing using two-photon light sheet microscopy and multicore fibre illumination;
  • small animals in vivo testing of fast two-photon imaging using a miniaturised probe head;
  • quantitative analysis of two-photon images enables diagnosis of disease.

Part of this PhD project will be conducted in collaboration with Dr. Esben Andresen from the University of Lille (France) and Prof. Kishan Dholakia from the University of St Andrews (Scotland), and the successful candidate will collaborate with these two groups. The PhD project is partly funded by the H2020 project PROSCOPE, and the successful candidate will collaborate with relevant project partners.

Qualifications
Candidates should have a two-year master's degree (120 ECTS points) or a similar degree with an academic level equivalent to a two-year master's degree.

Approval and Enrolment
The scholarship for the PhD degree is subject to academic approval, and the candidate will be enrolled in one of the general degree programmes at DTU. For information about our enrolment requirements and the general planning of the PhD study programme, please see the DTU PhD Guide . 

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

Salary and appointment terms
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union. The period of employment is 3 years. 

The initial workplace is DTU Risø Campus in Roskilde, Denmark.

You can read more about career paths at DTU here .

Further information
Further information may be obtained from Dominik Marti, tel.: +45 4677 4568, domar@dtu.dk , and Peter E. Andersen, tel.: +45 4677 4555, peta@dtu.dk .   

You can read more about DTU Health Technology at https://www.healthtech.dtu.dk . 

Application
Please submit your online application no later than 19 November 2020 (local time)Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

  • A letter motivating the application (cover letter)
  • Curriculum vitae
  • Grade transcripts and BSc/MSc diploma
  • Excel sheet with translation of grades to the Danish grading system (see guidelines and Excel spreadsheet here )

Candidates may apply prior to obtaining their master's degree but cannot begin before having received it.

Applications and enclosures received after the deadline will not be considered.

All interested candidates irrespective of age, gender, race, disability, religion or ethnic background are encouraged to apply.

DTU Health Tech engages in research, education, and innovation base on technical and natural science for the healthcare sector. The Healthcare sector is a globally expanding market with demands for the most advanced technological solutions. DTU Health Tech creates the foundation for companies to develop new and innovative services and products which benefit people and create value for society. DTU Health Techs expertise spans from imaging and biosensor techniques, across digital health and biological modelling, to biopharma technologies.The department has a scientific staff of about 175 persons, 130 PhD students and a technical/administrative support staff of about 80 persons.

Technology for people
With our international elite research and study programmes, DTU is helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 12,000 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. Our main campus is in Kgs. Lyngby north of Copenhagen and we have campuses in Roskilde and Ballerup and in Sisimiut in Greenland.


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