Supervisor: H. Robert Boisivon
Annotation:
Transcript variants generated by alternative splicing (AS) of a precursor mRNA transcript expand the cellular protein catalog for the plant response to various stresses without the requirement of de novo transcription. AS is a molecular strategy taken by plants under temperature stress to limit the adverse effects of high temperatures and to adjust their growth and physiology during the stress period. Spliced variants may be targeted for degradation by non-sense mediated decay (NMD) or encode alternative proteins to feedback on the stress response. Heat Shock Proteins (HSP) and Heat Shock Factors (HSF) are among the targets of temperature-induced AS. Our lab investigates how high temperatures affect embryo morphogenesis and seed development. We identified that AS regulators are upregulated in seeds of plants grown at high temperatures. The project will investigate the implications of AS in the morphogenic alterations of Arabidopsis embryos when developing at high temperatures. The proposed experimental approaches include profiling of AS events in high-temperature seeds by nanopore RNA sequencing. Specific candidates whose transcripts are targeted by AS at high temperatures, and known for their involvement in embryo morphogenesis, will be functionally characterized: expression analysis (microscopy), phenotyping (genetics), ectopic expression (cloning, generation of transgenics), etc.
The Ph.D. topic is part of a collaborative project with the research team led by Dr. Said Hafidh in the lab of Prof. Honys at the Institute of Experimental Botany in Prague.
Recommended literature:
John, S., Olas, J. J. & Roeber, B. M. Regulation of alternative splicing in response to temperature variation in plants. J Exp Bot erab232 (2021) doi:10.1093/jxb/erab232.
Kulichová, K. et al. PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana. Development (Cambridge, England) 147, dev186742 (2020).
Mácová, K. et al. Effects of long-term high-temperature stress on reproductive growth and seed development in development in Brassica napus. Biorxiv 2021.03.11.434971 (2021) doi:10.1101/2021.03.11.434971.
Robert, H. S. et al. Local auxin sources orient the apical-basal axis in Arabidopsis embryos. Current Biology 23, 2506–2512 (2013).
Verma, S., Attuluri, V. P. S. & Robert, H. S. An Essential Function for Auxin in Embryo Development. Csh Perspect Biol 13, a039966 (2021).
For more detail about our group, please refer to our website: http://boisivonlab.ceitec.cz . The team currently has 11 members (5 Ph.D. students, three postdocs, three lab technicians). We are international (Czech, French, Croat, Indian, Lebanese, Iranian, Spanish).
Applications should include: a CV, a scanned copy of the Masters or an official letter certifying its completion, brief motivation letter, and contacts for at least two references should be sent via e-mail [email protected] or [email protected] .
More information about CEITEC PhD School are available at http://ls-phd.ceitec.cz /.
Similar Positions
-
Ph D Student: Machine Learning In Plant Regulatory Genomics, VIB, Belgium, 1 day ago
Job description The Vandepoele research lab (Comparative Network Biology - https://www.vandepoelelab.be/ ) invites applications for a fully-funded PhD project for 1 year and extended with another...
-
Ph D Position In Experimental Physics With Focus On Optical Frequency Comb Spectroscopy , Umeå University, Sweden, about 24 hours ago
Umeå University is one of Sweden’s largest higher education institutions with over 37,000 students and about 4,700 employees. The University offers a diversity of high-quality education and world-...
-
Ph D Scholarship In Magnetothermal Energy Harvesting From Waste Heat, European Magnetism Association EMA, Denmark, 22 days ago
Description of the offer : Do you want to contribute to a future where we can harvest energy from waste heat using a novel magnetothermal device, that you will get to help realize? If yes, this Ph...
-
Ph D Candidate In Integrated Modeling For Plant Life History Traits , University of Amsterdam, Netherlands, 20 days ago
Do you enjoy using computational approaches to study biological problems? Are you interested in using a combination of machine learning and mechanistic modelling? We invite enthusiastic and dedica...
-
Ph D Candidate In Airflow Distribution, Heat/Mass Transfer And Ventilation In Hospital Environments , NTNU - Norwegian University of Science and Technology, Norway, 1 day ago
24th April 2024 Languages English English English The Department of Energy and Process Engineering has a vacancy for a PhD candidate in airflow distribution, heat/mass transfer and ventilation in ...
-
Ph D In The Field Of Nmr Supported Amyloid And Bio Condensate Structural Biology , Technical University of Munich, Germany, 1 day ago
08.04.2024, Wissenschaftliches Personal The Reif group at the Bavarian NMR Center (BNMRZ) at the TU Munich is looking for a PhD student to work on the characterization of amyloid fibrils, protein ...